Mouse Models of Innate Immunity

Methods and Protocols

  • Irving C. Allen

Part of the Methods in Molecular Biology book series (MIMB, volume 1031)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Albert G. Zimmermann, Yue Sun
    Pages 1-18
  3. Freddy M. Sanchez, German I. Cuadra, Stanton J. Nielsen, Anne Tanner, Bradford K. Berges
    Pages 19-26
  4. Emilie Imbeault, Denis Gris
    Pages 51-57
  5. Chris B. Moore, Irving C. Allen
    Pages 65-70
  6. Kelly Roney
    Pages 71-76
  7. Kristin M. Long, Mark Heise
    Pages 97-100
  8. Irving C. Allen
    Pages 101-107
  9. Natalia Malachowa, Scott D. Kobayashi, Kevin R. Braughton, Frank R. DeLeo
    Pages 109-116
  10. Nancy Wang, Richard A. Strugnell, Odilia L. Wijburg, Thomas C. Brodnicki
    Pages 125-144
  11. Donna M. MacCallum
    Pages 145-153
  12. Umesh C. S. Yadav, Kota V. Ramana
    Pages 155-162
  13. Irving C. Allen
    Pages 163-175
  14. Catherine J. Sanders, Brian Johnson, Charles W. Frevert, Paul G. Thomas
    Pages 177-188
  15. Joshua Uronis, Xiaolun Sun
    Pages 197-202
  16. Marcela Montes de Oca, Christian Engwerda, Ashraful Haque
    Pages 203-213
  17. Back Matter
    Pages 223-225

About this book


The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.


Disease process assessment Host innate immune response Mouse genetics Mouse models Primary cells

Editors and affiliations

  • Irving C. Allen
    • 1
  1. 1., Dept. of Biomedical Sciences & PathobiolVirginia TechBlacksburgUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-480-7
  • Online ISBN 978-1-62703-481-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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