Guanylate Cyclase and Cyclic GMP

Methods and Protocols

  • Thomas Krieg
  • Robert Lukowski

Part of the Methods in Molecular Biology book series (MIMB, volume 1020)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michael Russwurm, Corina Russwurm, Doris Koesling, Evanthia Mergia
    Pages 1-16
  3. Franz Hofmann, Jörg W. Wegener
    Pages 17-50
  4. Sergei D. Rybalkin, Thomas R. Hinds, Joseph A. Beavo
    Pages 51-62
  5. Ronald Jäger, Dieter Groneberg, Barbara Lies, Noomen Bettaga, Michaela Kümmel, Andreas Friebe
    Pages 63-72
  6. Thomas C. Rich, Andrea L. Britain, Tiffany Stedman, Silas J. Leavesley
    Pages 73-88
  7. Martin Thunemann, Natalie Fomin, Christian Krawutschke, Michael Russwurm, Robert Feil
    Pages 89-120
  8. Konrad R. Götz, Viacheslav O. Nikolaev
    Pages 121-129
  9. Katharina Salb, Jens Schlossmann
    Pages 147-162
  10. Joseph Robert Burgoyne, Philip Eaton
    Pages 163-173
  11. Katja Jennissen, Bodo Haas, Michaela M. Mitschke, Franziska Siegel, Alexander Pfeifer
    Pages 175-192
  12. Hannes Schmidt, Gohar Ter-Avetisyan, Fritz G. Rathjen
    Pages 193-204
  13. Peter M. Schmidt, Johannes-Peter Stasch
    Pages 205-214
  14. Stylianos Michalakis, Jianhua Xu, Martin Biel, Xi-Qin Ding
    Pages 235-245
  15. Back Matter
    Pages 247-249

About this book


Recently, many ground-breaking steps have been made towards  better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.




Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide  scientist current methods and a useful guide towards the possibility to apply these techniques to their own research.


Cyclic GMP Guanylate Cyclase NO/cGMP/PKG pathway cellular level localisation nitric oxide patho-/physiological integration physiological and phathophysiological conditions signalling molecule whole animal models

Editors and affiliations

  • Thomas Krieg
    • 1
  • Robert Lukowski
    • 2
  1. 1.Addenbrooke’s Hospital, Department of MedicineUniversity of CambridgeCambridgeUnited Kingdom
  2. 2.Toxicology and Clinical Pharmacy, Department of PharmacologyUniversität TübingenTübingenGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-458-6
  • Online ISBN 978-1-62703-459-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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