Chemical Neurobiology

Methods and Protocols

  • Matthew R. Banghart

Part of the Methods in Molecular Biology book series (MIMB, volume 995)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Chemical Probes of Membrane Protein Structure and Function

    1. Front Matter
      Pages 1-1
    2. Alexander G. Komarov, Corey A. Costantino, Francis I. Valiyaveetil
      Pages 3-17
    3. Sviatoslav N. Bagriantsev, Daniel L. Minor Jr.
      Pages 31-42
    4. Jessica H. Harvey, Richard M. van Rijn, Jennifer L. Whistler
      Pages 43-54
  3. Photochemical Control of Protein and Cellular Function

    1. Front Matter
      Pages 55-55
    2. Alexandre Specht, Frederic Bolze, Jean Francois Nicoud, Maurice Goeldner
      Pages 79-87
    3. Alexandre Mourot, Timm Fehrentz, Richard H. Kramer
      Pages 89-105
    4. Pamela M. England
      Pages 121-129
  4. Chemical Probes for Imaging in the Nervous System

  5. Back Matter
    Pages 207-219

About this book


Many advances in modern neuroscience are enabled by the availability of chemical tools that allow sensitive, precise, and quantitative measurements of, and control over, biological processes. These powerful reagents are widely used for investigating the nervous system at levels of detail ranging from ion channel structure to neural network dynamics. Recent advances in photochemistry, microscopy, and protein engineering have triggered a surge in the development and application of these interdisciplinary techniques. Chemical Neurobiology: Methods and Protocols assists with the design, characterization and validation of new chemical tools for neurobiology by providing detailed protocols of procedures and assays deemed essential for the successful development and implementation of such tools. Divided into three sections, topics cover chemical probes of membrane protein structure and function, photochemical control of protein and cellular function, and chemical probes for imaging in the nervous system. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Chemical Neurobiology: Methods and Protocols serves scientists at many levels, including students aiming to expand their perspective, laboratory researchers seeking technical guidance, and established investigators looking for creative solutions to their research problems in molecular, cellular and systems neuroscience. 


GPCR Heterodimers flash photolysis fluorescent ion sensors ion channel complexes membrane protein structure peptide toxins photoreactive antagonist potassium channel function

Editors and affiliations

  • Matthew R. Banghart
    • 1
  1. 1., Department of NeurobiologyHarvard Medical SchoolBostonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-344-2
  • Online ISBN 978-1-62703-345-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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