© 2013

Chiral Separations

Methods and Protocols

  • Gerhard K. E. Scriba

Part of the Methods in Molecular Biology book series (MIMB, volume 970)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Massimo Del Bubba, Leonardo Checchini, Alessandra Cincinelli, Luciano Lepri
    Pages 29-43
  3. Imran Ali, Zeid A. AL-Othman, Hassan Y. Aboul-Enein
    Pages 127-135
  4. Gregory K. Webster, Ted J. Szczerba
    Pages 177-190
  5. Benedetto Natalini, Roccaldo Sardella, Federica Ianni
    Pages 191-208
  6. Lushan Yu, Shengjia Wang, Su Zeng
    Pages 221-231

About this book


There is a demand for analytical methods that are able to discriminate between enantiomers in order to analyze the enantiomeric purity of compounds from natural or chemical sources not only in pharmaceutical sciences but in any field on bioactive compounds including chemistry, biology, biochemistry, forensic, and environmental sciences and many others. The second edition of Chiral Separations: Methods and Protocols, expands upon the previous edition with current methodology, providing an overview and especially practically oriented applications of the most important analytical techniques in chiral separation sciences. New chapters on analytical separation sciences by chromatographic and electrophoretic techniques have been added as has simulated moving bed chromatography as a preparative method. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Chiral Separations: Methods and Protocols, Second Edition is helpful for analytical chemists working on stereochemical problems in fields or pharmacy, chemistry, biochemistry, food chemistry, molecular biology, forensics, environmental sciences or cosmetics in academia, government or industry.


analytical procedures chiral separation chirality chromatographic techniques chromatography electrophoretic techniques enantioseparations physico-chemical properties stereochemistry

Editors and affiliations

  • Gerhard K. E. Scriba
    • 1
  1. 1., Department of Pharmaceutical ChemistryFriedrich-Schiller-University JenaJenaGermany

Bibliographic information

  • Book Title Chiral Separations
  • Book Subtitle Methods and Protocols
  • Editors Gerhard K. E. Scriba
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-62703-262-9
  • Softcover ISBN 978-1-4939-5974-7
  • eBook ISBN 978-1-62703-263-6
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XVII, 531
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site
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From the reviews of the second edition:

“The second edition of Chiral Separations: Methods and Protocols discusses almost every kind of chiral separation method, with the emphasis on fundamental research and experimental design. … it is a very useful book for scientists and students involved in the enantioseparation field, and scientists involved in R & D areas such as drug development and environmental monitoring can also benefit from this monograph. It is expected that this book will definitely promote research in chirality-related fields.” (Huwei Liu, Analytical and Bioanalytical Chemistry, Vol. 406, 2014)

“The book provides an overview and practically oriented applications of the most important analytical techniques in chiral separations. … the chapters include introductions, lists of necessary materials and reagents, step-by-step laboratory protocols, and troubleshooting tips. Pharmacists, biochemists, chemists, food chemists, molecular biologists, and forensic and environmental scientists will find this book extremely useful.” (Omer Iqbal, Doody’s Book Reviews, August, 2013)