Basic Cell Culture Protocols

  • Cheryl D. Helgason
  • Cindy L. Miller

Part of the Methods in Molecular Biology book series (MIMB, volume 946)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Cord C. Uphoff, Hans G. Drexler
    Pages 1-13
  3. Cord C. Uphoff, Hans G. Drexler
    Pages 15-26
  4. Roderick A. F. MacLeod, Hans G. Drexler
    Pages 39-60
  5. Takeshi Nitta, Izumi Ohigashi, Yousuke Takahama
    Pages 85-102
  6. Génève Awong, Juan Carlos Zúñiga-Pflücker
    Pages 103-113
  7. Aysegul V. Ergen, Mira Jeong, Kuanyin K. Lin, Grant A. Challen, Margaret A. Goodell
    Pages 151-162
  8. Craig Gedye, Laurie Ailles
    Pages 181-204
  9. Nicolas Pineault, Amélie Robert, Valérie Cortin, Lucie Boyer
    Pages 205-224
  10. Shelley B. Weisser, Keith W. McLarren, Etsushi Kuroda, Laura M. Sly
    Pages 225-239
  11. Min Liu, Cindy L. Miller, Connie J. Eaves
    Pages 241-256
  12. Stefan Woehrer, Cindy L. Miller, Connie J. Eaves
    Pages 257-266
  13. Bert Wognum, Ning Yuan, Becky Lai, Cindy L. Miller
    Pages 267-283
  14. Sean A. Parsons, Christophe Jurzinsky, Susan L. Cuvelier, Kamala D. Patel
    Pages 285-300
  15. Rouzanna Istvanffy, Robert A. J. Oostendorp
    Pages 301-314
  16. Johng S. Rhim
    Pages 383-393
  17. Michael Prater, Mona Shehata, Christine J. Watson, John Stingl
    Pages 395-409
  18. Ricarda Glaum, Margit Wiedmann-Al-Ahmad
    Pages 423-429
  19. Paul Keire, Andrew Shearer, Gabi Shefer, Zipora Yablonka-Reuveni
    Pages 431-468
  20. Takamichi Ishii, Kentaro Yasuchika, Iwao Ikai
    Pages 469-478
  21. Nirupama K. Shevde, Amber A. Mael
    Pages 535-546
  22. Back Matter
    Pages 547-550

About this book


At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems.  Written in the highly successful Methods in Molecular Biology™, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.


Cell culture systems Cell differentiation Cell isolation Cellular processes Specialized primary cell types Tissue culture techniques

Editors and affiliations

  • Cheryl D. Helgason
    • 1
  • Cindy L. Miller
    • 2
  1. 1., Experimental TherapeuticsBC Cancer AgencyVancouverCanada
  2. 2.STEMCELL Technologies, Inc.VancouverCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2013
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-127-1
  • Online ISBN 978-1-62703-128-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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