Single-Stranded DNA Binding Proteins

Methods and Protocols

  • James L. Keck

Part of the Methods in Molecular Biology book series (MIMB, volume 922)

Table of contents

  1. Front Matter
    Pages i-x
  2. Alexander G. Kozlov, Timothy M. Lohman
    Pages 37-54
  3. Alexander G. Kozlov, Roberto Galletto, Timothy M. Lohman
    Pages 55-83
  4. Chris A. Brosey, Susan E. Tsutakawa, Walter J. Chazin
    Pages 101-122
  5. Mikhail Ryzhikov, Sergey Korolev
    Pages 123-131
  6. Brian W. Walsh, Justin S. Lenhart, Jeremy W. Schroeder, Lyle A. Simmons
    Pages 161-168
  7. Asher N. Page, Nicholas P. George
    Pages 169-174
  8. Jin Inoue, Tsutomu Mikawa
    Pages 175-182
  9. Cathy S. Hass, Ran Chen, Marc S. Wold
    Pages 193-204
  10. Dusica Vujaklija, Boris Macek
    Pages 205-218
  11. Katy Hedgethorne, Martin R. Webb
    Pages 219-233
  12. Piero R. Bianco, Adam J. Stanenas, Juan Liu, Christopher S. Cohan
    Pages 235-244
  13. Back Matter
    Pages 255-259

About this book


The genomes of cellular organisms are organized as double-stranded DNA, a structure that must be unwound to provide DNA replication, recombination, and repair machinery access to genomic information.  However, DNA unwinding comes with inherent risks to genome stability.  To help mediate these risks, bacterial, archael, and eukaryotic cells have evolved protective ssDNA-binding proteins (SSBs) that bind ssDNA with high affinity and specificity.  SSBs also aid genome metabolic processes through direct interactions with key proteins in genome maintenance enzymes.  Single-Stranded DNA Binding Proteins: Methods and Protocols assembles methods developed for examining the fundamental properties of SSBs and for exploiting the biochemical functions of SSBs for their use as in vitro and in vivo reagents. Clearly and concisely organized, the volume opens with an introduction to the structures and functions of SSBs, followed protocols for studying SSB/DNA complexes, methods for studying SSB/heterologous protein complexes, protocols for interrogating post-translational modifications of SSBs, and concludes with uses of fluorescently-labeled SSBs for in vitro and in vivo studies of genome maintenance processes.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Single-Stranded DNA Binding Proteins: Methods and Protocols provides a rich introduction for investigators who are interested in this fascinating family of DNA-binding proteins.


DNA-binding proteins SSB proteins genome maintenance enzymes heterologous protein complexes ssDNA

Editors and affiliations

  • James L. Keck
    • 1
  1. 1.School of Medicine and Public Health, Department of Biomolecular ChemistryUniversity of WisconsinMadisonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-62703-031-1
  • Online ISBN 978-1-62703-032-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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