High-Throughput Phenotyping in Plants

Methods and Protocols

  • Jennifer Normanly

Part of the Methods in Molecular Biology book series (MIMB, volume 918)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Bettina Berger, Bas de Regt, Mark Tester
    Pages 9-20
  3. Nima Yazdanbakhsh, Joachim Fisahn
    Pages 21-40
  4. Roland Pieruschka, Denis Klimov, Joseph A. Berry, C. Barry Osmond, Uwe Rascher, Zbigniew S. Kolber
    Pages 51-59
  5. László Sass, Petra Majer, Éva Hideg
    Pages 61-69
  6. Tim Brown, Christopher Zimmermann, Whitney Panneton, Nina Noah, Justin Borevitz
    Pages 71-96
  7. Raju Naik Vankudavath, Reddaiah Bodanapu, Yellamaraju Sreelakshmi, Rameshwar Sharma
    Pages 97-116
  8. Christian Schudoma, Matthias Steinfath, Heike Sprenger, Joost T. van Dongen, Dirk Hincha, Ellen Zuther et al.
    Pages 127-150
  9. Will I. Menzel, Wen-Ping Chen, Adrian D. Hegeman, Jerry D. Cohen
    Pages 165-178
  10. Thilani Samarakoon, Sunitha Shiva, Kaleb Lowe, Pamela Tamura, Mary R. Roth, Ruth Welti
    Pages 179-268
  11. Carlo Bicchi, Massimo Maffei
    Pages 289-310
  12. David W. Galbraith, Georgina M. Lambert
    Pages 311-325
  13. Scott J. Lee, Thomas A. Warnick, Susan B. Leschine, Samuel P. Hazen
    Pages 341-349
  14. Jonatan U. Fangel, Henriette L. Pedersen, Silvia Vidal-Melgosa, Louise I. Ahl, Armando Asuncion Salmean, Jack Egelund et al.
    Pages 351-362
  15. Back Matter
    Pages 363-365

About this book


Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High-Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, High-Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.


image capture morphometric analysis phenotype analysis plant development plant growth quantitative profiling visual screens

Editors and affiliations

  • Jennifer Normanly
    • 1
  1. 1., Dept. of Biochemistry & Molecular BiologUniversity of MassachusettsAmherstUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-994-5
  • Online ISBN 978-1-61779-995-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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