Xenopus Protocols

Post-Genomic Approaches

  • Peter D Vize

Part of the Methods in Molecular Biology book series (MIMB, volume 917)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Xenopus Tropicalis

    1. Front Matter
      Pages 1-1
    2. Alan Jafkins, Anita Abu-Daya, Anna Noble, Lyle B. Zimmerman, Matthew Guille
      Pages 17-31
    3. Florencia del Viso, Mustafa Khokha
      Pages 33-41
    4. Ira L. Blitz
      Pages 43-65
  3. Genetics in Xenopus

    1. Front Matter
      Pages 67-67
    2. Timothy J. Geach, Derek L. Stemple, Lyle B. Zimmerman
      Pages 69-110
    3. Donald A. Yergeau, Clair M. Kelley, Haiqing Zhu, Emin Kuliyev, Paul E. Mead
      Pages 111-127
    4. John J. Young, Richard M. Harland
      Pages 129-141
    5. Yanan Zhao, Shoko Ishibashi, Enrique Amaya
      Pages 143-154
    6. Matthew L. Tomlinson, Adam E. Hendry, Grant N. Wheeler
      Pages 155-166
  4. Xenopus Trangenesis

    1. Front Matter
      Pages 183-183
    2. Shoko Ishibashi, Kristen L. Kroll, Enrique Amaya
      Pages 185-203
    3. Shoko Ishibashi, Nick R. Love, Enrique Amaya
      Pages 205-218
    4. You E. Li, Bryan G. Allen, Daniel L. Weeks
      Pages 219-230
    5. Clair M. Kelley, Donald A. Yergeau, Haiqing Zhu, Emin Kuliyev, Paul E. Mead
      Pages 231-243
    6. Hajime Ogino, Haruki Ochi, Chihiro Uchiyama, Sarah Louie, Robert M. Grainger
      Pages 245-263
  5. Gene Expression: From Gene to Transcript to Protein

    1. Front Matter
      Pages 277-277
    2. Robert C. Akkers, Ulrike G. Jacobi, Gert Jan C. Veenstra
      Pages 279-292
    3. Dasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faunes, Francisco Melo, Juan Larrain
      Pages 293-317
    4. Michael J. Gilchrist, Nicolas Pollet
      Pages 319-345
    5. Agnès Mereau, Serge Hardy
      Pages 347-368
    6. Frank L. Conlon, Yana Miteva, Erin Kaltenbrun, Lauren Waldron, Todd M. Greco, Ileana M. Cristea
      Pages 369-390
    7. Todd M. Greco, Yana Miteva, Frank L. Conlon, Ileana M. Cristea
      Pages 391-407
  6. Imaging Xenopus Development

    1. Front Matter
      Pages 409-409
    2. Eamon Dubaissi, Niki Panagiotaki, Nancy Papalopulu, Peter D. Vize
      Pages 411-429
    3. Robert Lea, Boyan Bonev, Eamon Dubaissi, Peter D. Vize, Nancy Papalopulu
      Pages 431-444
    4. Hélène Auger, Raphaël Thuret, Warif El Yakoubi, Nancy Papalopulu
      Pages 461-475
    5. Sagar D. Joshi, Hye Young Kim, Lance A. Davidson
      Pages 477-493
  7. Novel Approaches in Xenopus

    1. Front Matter
      Pages 495-495
    2. Caroline W. Beck
      Pages 525-539
    3. Jeff Bowes
      Pages 541-562
  8. Back Matter
    Pages 563-568

About this book


During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of  Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.  


Xenopus Xenopus laevis Xenopus tropicalis conserved vertebrate genomic eggs embryos fertilization genome protein expression proteome level vertebrate genomes

Editors and affiliations

    • 1
  • Peter D Vize
    • 2
  1. 1., Institute of Medical SciencesUniversity of AberdeenABERDEENUnited Kingdom
  2. 2., Department of Biological ScienceUniversity of CalgaryCalgaryCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-991-4
  • Online ISBN 978-1-61779-992-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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