Embryo Culture

Methods and Protocols

  • Gary D. Smith
  • Jason E. Swain
  • Thomas B. Pool

Part of the Methods in Molecular Biology book series (MIMB, volume 912)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jacques Cohen, Don Rieger
    Pages 1-18
  3. Calvin Simerly, Gerald Schatten
    Pages 39-59
  4. Jay M. Baltz
    Pages 61-80
  5. Deirdre Zander-Fox, Michelle Lane
    Pages 81-96
  6. Franchesca D. Houghton
    Pages 97-106
  7. Catherine M. H. Combelles, Margo L. Hennet
    Pages 129-159
  8. Jason E. Swain
    Pages 161-175
  9. Aparna Hegde, Barry Behr
    Pages 177-198
  10. Ronit Machtinger, Catherine Racowsky
    Pages 199-209
  11. Patrick Quinn
    Pages 211-230
  12. Yves J. R. Ménézo, Edouard Servy, Anna Veiga, André Hazout, Kay Elder
    Pages 231-247
  13. Borut Kovačič
    Pages 249-272
  14. Michael L. Reed
    Pages 273-312
  15. Theodore Thomas
    Pages 313-324
  16. Dean E. Morbeck, Phoebe H. Leonard
    Pages 325-331
  17. André Monteiro da Rocha, Gary D. Smith
    Pages 355-365
  18. Thomas B. Pool, John Schoolfield, David Han
    Pages 367-386
  19. Brenna A. Market Velker, Michelle M. Denomme, Mellissa R. W. Mann
    Pages 399-421
  20. Back Matter
    Pages 423-433

About this book


Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods  and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.


cell function embryo development environmental influence epigenetics human embryo in vitro fertilization infertility reproductive technologies

Editors and affiliations

  • Gary D. Smith
    • 1
  • Jason E. Swain
    • 2
  • Thomas B. Pool
    • 3
  1. 1., OB/GYNUniversity of MichiganAnn ArborUSA
  2. 2., Center for Reproductive MedicineUniversity of MichiganAnn ArborUSA
  3. 3.Fertility Center of San AntonioSan AntonioUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-970-9
  • Online ISBN 978-1-61779-971-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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