Single Domain Antibodies

Methods and Protocols

  • Dirk Saerens
  • Serge Muyldermans

Part of the Methods in Molecular Biology book series (MIMB, volume 911)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Overview of Single Domain Antibodies

    1. Front Matter
      Pages 1-1
    2. Cécile Vincke, Serge Muyldermans
      Pages 15-26
  3. Single Domain Antibody Library Construction

    1. Front Matter
      Pages 37-37
    2. Olga Ignatovich, Laurent Jespers, Ian M. Tomlinson, Ruud M. T. de Wildt
      Pages 39-63
    3. Aurelien Olichon, Ario de Marco
      Pages 65-78
  4. Selection of Single Domain Antibodies

    1. Front Matter
      Pages 79-79
    2. Kristof De Schutter, Nico Callewaert
      Pages 125-134
    3. Mireille Pellis, Serge Muyldermans, Cécile Vincke
      Pages 135-150
    4. Tomoyuki Tanaka, Terence H. Rabbitts
      Pages 151-173
    5. Edward Dolk, Theo Verrips, Hans de Haard
      Pages 199-209
    6. Greg Hussack, Mehdi Arbabi-Ghahroudi, C. Roger MacKenzie, Jamshid Tanha
      Pages 211-239
    7. Rinse Klooster, Kim S. Rutgers, Silvère M. van der Maarel
      Pages 241-253
  5. Expression of Single Domain Antibodies and Derivatives

    1. Front Matter
      Pages 225-225
    2. Toya Nath Baral, Mehdi Arbabi-Ghahroudi
      Pages 257-275
    3. Andrea Gorlani, Hans de Haard, Theo Verrips
      Pages 277-286
    4. Vishal Agrawal, Igor Slivac, Sylvie Perret, Louis Bisson, Gilles St-Laurent, Yanal Murad et al.
      Pages 287-303
    5. Sylvie De Buck, Vikram Virdi, Thomas De Meyer, Kirsten De Wilde, Robin Piron, Jonah Nolf et al.
      Pages 305-324
  6. Improvement and Applications of Single Domain Antibodies

    1. Front Matter
      Pages 325-325
    2. Raffi Tonikian, Sachdev S. Sidhu
      Pages 327-353
    3. Dae Young Kim, Wen Ding, Jamshid Tanha
      Pages 355-372
    4. Lucy Rutten, Hans de Haard, Theo Verrips
      Pages 373-381
    5. Kip Dudgeon, Romain Rouet, Kristoffer Famm, Daniel Christ
      Pages 383-397
    6. Greg Hussack, C. Roger MacKenzie, Jamshid Tanha
      Pages 417-429
    7. Jianbing Zhang, C. Roger MacKenzie
      Pages 445-456
    8. Catarina Xavier, Nick Devoogdt, Sophie Hernot, Ilse Vaneycken, Matthias D’Huyvetter, Jens De Vos et al.
      Pages 485-490
    9. Trong Nguyen Duc, Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, Eveline Peeters, Daniel Charlier, Serge Muyldermans
      Pages 491-505
    10. Kien T. Maik, C. Roger MacKenzie
      Pages 523-530
  7. Case Studies

    1. Front Matter
      Pages 531-531
    2. Erwin De Genst, Christopher M. Dobson
      Pages 533-558
    3. Nick Devoogdt, Catarina Xavier, Sophie Hernot, Ilse Vaneycken, Matthias D’Huyvetter, Jens De Vos et al.
      Pages 559-567
    4. Kourosh Zolghadr, Jacqueline Gregor, Heinrich Leonhardt, Ulrich Rothbauer
      Pages 569-575
  8. Back Matter
    Pages 577-580

About this book


The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Heavy chain antibodies Single variable domain V-NAR VHH sdAbs

Editors and affiliations

  • Dirk Saerens
    • 1
  • Serge Muyldermans
    • 2
  1. 1., Molecular & Cellular InteractionsVrije Universiteit BrusselBrusselsBelgium
  2. 2., Molecular & Cellular InteractionsVrije Universiteit BrusselBrusselsBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-967-9
  • Online ISBN 978-1-61779-968-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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