Table of contents

  1. Front Matter
    Pages i-x
  2. Hilmar Ebersbach, Gabriele Proetzel, Chonghui Zhang
    Pages 1-10
  3. C. Ronald Geyer, John McCafferty, Stefan Dübel, Andrew R. M. Bradbury, Sachdev S. Sidhu
    Pages 11-32
  4. Konstantin Petropoulos
    Pages 33-51
  5. George Thom, Maria Groves
    Pages 101-116
  6. Brice Laffleur, Virginie Pascal, Christophe Sirac, Michel Cogné
    Pages 149-159
  7. Michael Schräml, Matthias Biehl
    Pages 171-181
  8. Michael Schräml, Leopold von Proff
    Pages 183-194
  9. Christiane Jäger, Claudia Ferrara, Pablo Umaña, Anne Zeck, Jörg Thomas Regula, Hans Koll
    Pages 195-208
  10. Simona Jevševar, Mateja Kusterle, Maja Kenig
    Pages 233-246
  11. Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, Ulrich Brinkmann
    Pages 247-263
  12. Alexander K. Haas, Klaus Mayer, Ulrich Brinkmann
    Pages 265-276
  13. Virginie Pascal, Brice Laffleur, Michel Cogné
    Pages 295-317
  14. Hilmar Ebersbach, Gabriele Proetzel
    Pages E1-E1
  15. Back Matter
    Pages 319-325

About this book


The rapidly growing field of antibody research is the result of many advancing technologies allowing current developments to take advantage of molecular engineering to create tailor-made antibodies.  Antibody Methods and Protocols attempts to provide insight into the generation of antibodies using in vitro and in vivo approaches, as well as technical aspects for screening, analysis, and modification of antibodies and antibody fragments.  The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and easy to use, Antibody Methods and Protocols provides a broad and useful background to support ongoing efforts by novices and experts alike and encourages the development of new imaginative approaches to this vital area of study.


Antibodies Antibody drug conjugates Modification Screening and analysis Therapeutics

Editors and affiliations

  • Gabriele Proetzel
    • 1
  • Hilmar Ebersbach
    • 2
  1. 1.The Jackson LaboratoryBar HarborUSA
  2. 2.Novartis Pharma AGBaselSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-930-3
  • Online ISBN 978-1-61779-931-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals