Therapeutic Proteins

Methods and Protocols

  • Vladimir Voynov
  • Justin A. Caravella

Part of the Methods in Molecular Biology book series (MIMB, volume 899)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Dimiter S. Dimitrov
    Pages 1-26
  3. Bryce Nelson, Sachdev S. Sidhu
    Pages 27-41
  4. Damjana Kastelic, Mingyue He
    Pages 61-72
  5. Qi Zhao, Zhongyu Zhu, Dimiter S. Dimitrov
    Pages 73-84
  6. Joachim Feldwisch, Vladimir Tolmachev
    Pages 103-126
  7. Enrico DiGiammarino, Tariq Ghayur, Junjian Liu
    Pages 145-156
  8. Lili Mao, Masayori Inouye
    Pages 177-185
  9. Sabine Geisse, Bernd Voedisch
    Pages 203-219
  10. Thomas Jostock, Hans-Peter Knopf
    Pages 227-238
  11. Cory L. Nykiforuk, Joseph G. Boothe
    Pages 239-264
  12. Adam J. Lowe, Cameron L. Bardliving, Carl A. Batt
    Pages 265-275
  13. Margaret Doherty, Ciara A. McManus, Rebecca Duke, Pauline M. Rudd
    Pages 293-313
  14. Sujatha Gomathinayagam, Erik Hoyt, Alissa M. Thompson, Eric Brown, Khanita Karaveg, Stephen R. Hamilton et al.
    Pages 315-323
  15. Natalia V. Artemenko, Andrew G. McDonald, Gavin P. Davey, Pauline M. Rudd
    Pages 325-350
  16. Satish K. Singh, Maria R. Toler
    Pages 379-401
  17. Patrick M. Buck, Sandeep Kumar, Xiaoling Wang, Neeraj J. Agrawal, Bernhardt L. Trout, Satish K. Singh
    Pages 425-451
  18. Troy Cellmer, Nicolas L. Fawzi
    Pages 453-470
  19. Pedro Fonte, José Carlos Andrade, Vítor Seabra, Bruno Sarmento
    Pages 471-487
  20. Back Matter
    Pages 499-502

About this book


After the identification of a potential protein drug, the next critical step is the production of sufficient authentic material for testing, characterization, and clinical trials, which, when successful, leads to the need for robust methodologies for large-scale production, purification, characterization, viral inactivation, and continued testing of the final protein product.  Building on the valuable first edition, Therapeutic Proteins: Methods and Protocols, Second Edition aims to cover each of these key aspects of protein drug production through the contributions of authors from highly esteemed industrial and academic institutions around the world.  Emphasizing the newest developments in the field, this second edition also includes additional emphasis on discovery, including new display and screening methods as well as the design and engineering of new types of therapeutic proteins.  There is also discussion of computational and bioinformatics methods, and chapters on safety aspects of therapeutic protein development.  Written in the highly successful Methods in Molecular Biology™ format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Fully updated and practical, Therapeutic Proteins: Methods and Protocols, Second Edition provides an essential resource to all scientists working in the field of therapeutic proteins.


Bioinformatics Drug discovery Pharmaceutical design Protein drug production Protein therapeutics Therapeutic protein development

Editors and affiliations

  • Vladimir Voynov
    • 1
  • Justin A. Caravella
    • 2
  1. 1.MedImmune, LLCGaithersburgUSA
  2. 2., Department of Physical BiochemistryBiogen IdecCambridgeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-920-4
  • Online ISBN 978-1-61779-921-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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