Vaccinia Virus and Poxvirology

Methods and Protocols

  • Stuart N. Isaacs

Part of the Methods in Molecular Biology book series (MIMB, volume 890)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Chad R. Irwin, Andrew Farmer, David O. Willer, David H. Evans
    Pages 23-35
  3. Melanie Kremer, Asisa Volz, Joost H. C. M. Kreijtz, Robert Fux, Michael H. Lehmann, Gerd Sutter
    Pages 59-92
  4. Juana M. Sánchez-Puig, María M. Lorenzo, Rafael Blasco
    Pages 93-111
  5. Cheng-Yen Huang, Wen Chang
    Pages 123-133
  6. Subuhi Sherwani, Niamh Blythe, Laura Farleigh, Joachim J. Bugert
    Pages 135-146
  7. Leon C. W. Lin, Stewart A. Smith, David C. Tscharke
    Pages 147-159
  8. David Esteban, Scott Parker, Jill Schriewer, Hollyce Hartzler, R. Mark Buller
    Pages 177-198
  9. Inge E. A. Flesch, Yik Chun Wong, David C. Tscharke
    Pages 199-218
  10. Melissa Da Silva, Chris Upton
    Pages 233-258
  11. Florentina Rus, Kayla Morlock, Neal Silverman, Ngoc Pham, Girish J. Kotwal, William L. Marshall
    Pages 273-288
  12. Back Matter
    Pages 327-332

About this book


Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military personnel and relatively large numbers of U.S. civilians. Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.


Vaccinia virus in vivo models infectious disease poxvirology poxvirus bioinformatics poxvirus immunomodulatory proteins poxvirus pathogenesis recombinant viruses

Editors and affiliations

  • Stuart N. Isaacs
    • 1
  1. 1.School of Medicine and the Philadelphia, Division of Infectious DiseaseUniversity of PennsylvaniaPhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-875-7
  • Online ISBN 978-1-61779-876-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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