Prenatal Gene Therapy

Concepts, Methods, and Protocols

  • Charles Coutelle
  • Simon N. Waddington

Part of the Methods in Molecular Biology book series (MIMB, volume 891)

Table of contents

  1. Front Matter
    Pages i-x
  2. Charles Coutelle, Simon N. Waddington
    Pages 1-7
  3. Anna L. David, Simon N. Waddington
    Pages 9-39
  4. Raul Alba, Andrew H. Baker, Stuart A. Nicklin
    Pages 55-84
  5. Suet Ping Wong, Orestis Argyros, Richard P. Harbottle
    Pages 133-167
  6. Jessica L. Roybal, Pablo Laje, Jesse D. Vrecenak, Alan W. Flake
    Pages 169-181
  7. Vedanta Mehta, Donald Peebles, Anna L. David
    Pages 183-200
  8. Jessica L. Roybal, Masayuki Endo, Suzanne M. K. Buckley, Bronwen R. Herbert, Simon N. Waddington, Alan W. Flake
    Pages 201-218
  9. Khalil N. Abi-Nader, Michael Boyd, Alan W. Flake, Vedanta Mehta, Donald Peebles, Anna L. David
    Pages 219-248
  10. Citra N. Mattar, Arijit Biswas, Mahesh Choolani, Jerry K. Y. Chan
    Pages 249-271
  11. Juliette M. K. M. Delhove, Ahad A. Rahim, Tristan R. McKay, Simon N. Waddington, Suzanne M. K. Buckley
    Pages 273-290
  12. Vedanta Mehta, Khalil N. Abi-Nader, David Carr, Jacqueline Wallace, Charles Coutelle, Simon N. Waddington et al.
    Pages 291-328
  13. Back Matter
    Pages 389-392

About this book


The emerging field of prenatal gene therapy is founded on scientific and technical advances in fetal medicine, molecular biology and gene therapy. This preclinical research subject aims at applying gene therapy during pregnancy for the prevention of human diseases caused by early onset congenital or gestation related conditions.  In Prenatal Gene Therapy: Concepts, Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study gene therapy, fetal medicine and medical ethics. These include detailed protocols for vector production, for breeding and husbandry of the animal models, for  the surgical procedures of gene delivery in large and small animals and for the methods of gene transfer analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Thorough and intuitive, Prenatal Gene Therapy: Concepts, Methods and Protocols seeks to aid scientists in the further study of prenatal disease and gene therapy projects beyond the scope of fetal medicine.


congenital conditions fetal medicine gene delivery gene therapy gene transfer analysis gestation conditions molecular biology pregnancy prenatal gene therapy vector production

Editors and affiliations

  • Charles Coutelle
    • 1
  • Simon N. Waddington
    • 2
  1. 1.National Heart & Lung Inst., Molecular & Cellular Medicine Sec.Imperial College LondonLondonUnited Kingdom
  2. 2.Reader in Gene Transfer Technology, Gene Transfer Technology GroupUniversity College LondonLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC. 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-872-6
  • Online ISBN 978-1-61779-873-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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