Methods and Protocols

  • Chrissa Kioussi

Part of the Methods in Molecular Biology book series (MIMB, volume 887)

Table of contents

  1. Front Matter
    Pages i-x
  2. Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, Chrissa Kioussi
    Pages 15-22
  3. Kateryna Kyrylkova, Sergiy Kyryachenko, Chrissa Kioussi, Mark Leid
    Pages 23-31
  4. Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, Chrissa Kioussi
    Pages 33-39
  5. Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, Chrissa Kioussi
    Pages 41-47
  6. Malgorzata M. Barczyk, Donald Gullberg, Anne Isine Bolstad
    Pages 49-57
  7. Keishi Otsu, Naoki Fujiwara, Hidemitsu Harada
    Pages 59-67
  8. Hans U. Luder, Margrit Amstad-Jossi
    Pages 81-93
  9. Harald Osmundsen, Anne-Marthe Jevnaker, Maria A. Landin
    Pages 95-110
  10. Kentaro Akiyama, Chider Chen, Stan Gronthos, Songtao Shi
    Pages 111-121
  11. Shuhei Tsuchiya, Masaki J. Honda
    Pages 123-134
  12. Miguel Padial-Molina, Julie T. Marchesan, Andrei D. Taut, Qiming Jin, William V. Giannobile, Hector F. Rios
    Pages 135-148
  13. Masamitsu Oshima, Miho Ogawa, Masato Yasukawa, Takashi Tsuji
    Pages 149-165
  14. Javier Catón, Thimios A. Mitsiadis, Peter R. Morgan
    Pages 167-177
  15. Back Matter
    Pages 193-194

About this book


Only in recent times has the possibility of growing and implanting replacement teeth, made from one’s own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized.  In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field.  This detailed volume includes chapters on the detection of “tooth development” gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for trouble-shooting and avoiding known pitfalls.


Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a “bioengineered tooth” into the patient’s mouth.


Bioengineered teeth Developmental mechanism Gene perturbation Living tooth implants Odontogenesis Tooth development Tooth repair

Editors and affiliations

  • Chrissa Kioussi
    • 1
  1. 1.College of Pharmacy, Dept. of Pharmaceutical SciencesOregon State UniversityCorvallisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-859-7
  • Online ISBN 978-1-61779-860-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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