Table of contents

  1. Front Matter
    Pages i-xiii
  2. Hideo Matsumura, Naoya Urasaki, Kentaro Yoshida, Detlev H. Krüger, Günter Kahl, Ryohei Terauchi
    Pages 1-17
  3. Ankur R. Bhardwaj, Ritu Pandey, Manu Agarwal, Surekha Katiyar-Agarwal
    Pages 19-45
  4. Nadia Ponts, Duk-Won D. Chung, Karine G. Le Roch
    Pages 59-73
  5. Miin-Feng Wu, Doris Wagner
    Pages 75-86
  6. Marta Barcala, Carmen Fenoll, Carolina Escobar
    Pages 87-95
  7. Douglas W. Bryant Jr, Henry D. Priest, Todd C. Mockler
    Pages 97-110
  8. Lakshminarayan K. Venkatesh, Olufemi Fasina, David J. Pintel
    Pages 121-129
  9. Sun-Jung Kwon, Jang-Kyun Seo, A. L. N. Rao
    Pages 131-141
  10. Hongwei Zhao, Yifan Lii, Pei Zhu, Hailing Jin
    Pages 165-176
  11. Kashmir Singh, Ravneet Kaur, Wenping Qiu
    Pages 177-191
  12. Scott A. Givan, Christopher A. Bottoms, William G. Spollen
    Pages 201-219
  13. Back Matter
    Pages 229-231

About this book


RNA abundance analysis is one of the most important approaches for gene expression studies in the field of molecular biology. In RNA Abundance Analysis: Methods and Protocols, expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.  This volume includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, sulfur-containing RNAs, viral and satellite RNAs, RNA isoforms, and alternatively spliced RNA variants from various organisms, as well as key discussions of computational data processing for genome-wide datasets.  Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Essential and easy to use, RNA Abundance Analysis: Methods and Protocols provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and modified RNAs, which can assist you in your gene expression studies.


Computational data processing Gene expression studies Genome-wide next-generation sequencing RNA abundance analysis RNA expression mRNA isolation

Editors and affiliations

  • Hailing Jin
    • 1
  • Walter Gassmann
    • 2
  1. 1., Dept. Plant Pathology & MicrobiologyUniversity of CaliforniaRiversideUSA
  2. 2., Division of Plant SciencesUniversity of MissouriColumbiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-838-2
  • Online ISBN 978-1-61779-839-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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