In Vivo Cellular Imaging Using Fluorescent Proteins

Methods and Protocols

  • Robert M. Hoffman

Part of the Methods in Molecular Biology book series (MIMB, volume 872)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg, Jeffrey Wyckoff, John Condeelis et al.
    Pages 15-30
  3. Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, Mark W. Dewhirst
    Pages 31-50
  4. Hayao Nakanishi, Seiji Ito, Makoto Matsui, Yuichi Ito, Kazunari Misawa, Yasuhiro Kodera
    Pages 85-95
  5. Alexander P. Savitsky, Irina G. Meerovich, Victoria V. Zherdeva, Lyaysan R. Arslanbaeva, Olga S. Burova, Darina V. Sokolova et al.
    Pages 97-114
  6. Yong A. Yu, Stephanie Weibel, Aladar A. Szalay
    Pages 159-175
  7. Takashi Murakami, Eiji Kobayashi
    Pages 177-189
  8. Thomas E. McCann, Nobuyuki Kosaka, Peter L. Choyke, Hisataka Kobayashi
    Pages 191-204
  9. Irina V. Larina, Mary E. Dickinson
    Pages 205-215
  10. Fumitaka Takeshita, Ryou-u Takahashi, Jun Onodera, Takahiro Ochiya
    Pages 243-253
  11. Back Matter
    Pages 265-269

About this book


The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology.  What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.


3-dimensional in vivo imaging intravital imaging red fluorescent proteins tumor angiogenesis tumor cell invasion

Editors and affiliations

  • Robert M. Hoffman
    • 1
  1. 1.AntiCancer, Inc.San DiegoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-796-5
  • Online ISBN 978-1-61779-797-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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