Lipases and Phospholipases

Methods and Protocols

  • Georgina Sandoval

Part of the Methods in Molecular Biology book series (MIMB, volume 861)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Overview of Lipases, Phospholipases and Related Enzymes

    1. Front Matter
      Pages 1-1
    2. Leticia Casas-Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval, Alain Marty
      Pages 3-30
    3. Yassine Ben Ali, Robert Verger, Abdelkarim Abousalham
      Pages 31-51
    4. Mónica Sánchez-González, Allan Blanco-Gámez, Roberto Parra-Saldívar, Juan Carlos Mateos-Díaz, María Isabel Estrada-Alvarado
      Pages 53-61
    5. Ahmed Aloulou, Yassine Ben Ali, Sofiane Bezzine, Youssef Gargouri, Michael H. Gelb
      Pages 63-85
  3. Screening and Sources of Lipases, Phospholipases and Its Inhibitors

    1. Front Matter
      Pages 87-87
    2. Eduardo Mateos-Díaz, Jorge Alberto Rodríguez, María de los Ángeles Camacho-Ruiz, Juan Carlos Mateos-Díaz
      Pages 89-100
    3. Dolores Reyes-Duarte, Manuel Ferrer, Humberto García-Arellano
      Pages 101-113
    4. Ivanna Rivera, Juan Carlos Mateos-Díaz, Georgina Sandoval
      Pages 115-122
    5. Geliang Wang, Stephen Ryu, Xuemin Wang
      Pages 123-137
    6. Wallace Thompson, Rob C. Oslund, James Bollinger, Heather Ewing, Michael H. Gelb
      Pages 149-158
  4. Cloning, Production, Purification and Characterization of Lipases and Phospholipases

    1. Front Matter
      Pages 159-159
    2. Julian Gomez-Cambronero, Karen M. Henkels
      Pages 201-225
    3. Susana Velasco-Lozano, Tania Volke-Sepulveda, Ernesto Favela-Torres
      Pages 227-237
    4. Pablo Fuciños, Roberto González, Estrella Atanes, Ana Belén Fernández Sestelo, Nelson Pérez-Guerra, Lorenzo Pastrana et al.
      Pages 239-266
    5. Habib Horchani, Ahmed Fendri, Hanen Louati, Adel Sayari, Youssef Gargouri, Robert Verger
      Pages 267-282
    6. Aida Karray, Youssef Gargouri, Robert Verger, Sofiane Bezzine
      Pages 283-297
  5. Optimization of the Biocatalyst and Reaction Conditions

    1. Front Matter
      Pages 299-299
    2. Sophie Duquesne, Florence Bordes, Franck Fudalej, Jean-Marc Nicaud, Alain Marty
      Pages 301-312
    3. Christian C. Gruber, Jürgen Pleiss
      Pages 313-327
    4. Jose M. Palomo, Jose M. Guisan
      Pages 329-341
    5. Alberto del Monte-Martínez, Bessy V. Cutiño-Avila
      Pages 343-382
    6. Edmundo Castillo, Alejandro Torres-Gavilán, Georgina Sandoval, Alain Marty
      Pages 383-400
  6. Applications of Lipases and Phospholipases

    1. Front Matter
      Pages 401-401
    2. Ram Chandra Reddy Jala, Peng Hu, Tiankui Yang, Yuanrong Jiang, Yan Zheng, Xuebing Xu
      Pages 403-433
    3. Pamela Torres, Dolores Reyes-Duarte, Antonio Ballesteros, Francisco J. Plou
      Pages 435-443
    4. Alicia Baldessari
      Pages 445-456
    5. Xiaohu Fan, Xochitl Niehus, Georgina Sandoval
      Pages 471-483
    6. Karla A. Barrera-Rivera, Arturo Flores-Carreón, Antonio Martínez-Richa
      Pages 485-493
    7. Víctor Casado, Diana Martín, Carlos Torres, Guillermo Reglero
      Pages 495-523
    8. Enrique J. Herrera-López
      Pages 525-543
  7. Back Matter
    Pages 545-547

About this book


Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.


Lipases bio-physico-chemical enzymes heterologous expression inhibitors lipase/esterase concepts organic synthesis phospholipases plants thermodynamic tools

Editors and affiliations

  • Georgina Sandoval
    • 1
  1. 1.CIATEJ-CONACYTIndustrial Biotechnology UnitGuadalajaraMexico

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-599-2
  • Online ISBN 978-1-61779-600-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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