Pain Research

Methods and Protocols

  • Z. David Luo

Part of the Methods in Molecular Biology book series (MIMB, volume 851)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Z. David Luo
    Pages 1-8
  3. Dan Wang, Hyungsuk Kim, Xiao-Min Wang, Raymond Dionne
    Pages 9-46
  4. Rebecca M. Edelmayer, Michael H. Ossipov, Frank Porreca
    Pages 109-120
  5. David C. Yeomans, Mikhail Klukinov
    Pages 121-131
  6. Akiko Okada-Ogawa, Masamichi Shinoda, Kuniya Honda, Koichi Iwata
    Pages 133-148
  7. Kentaro Ono, Nozomu Harano, Kiyotoshi Inenaga, Osamu Nakanishi
    Pages 149-157
  8. Timothy K. Y. Kaan, Peter T. Ohara, Luc Jasmin
    Pages 159-170
  9. Julie Wieseler, Amanda Ellis, Steven F. Maier, Linda R. Watkins, Scott Falci
    Pages 171-183
  10. Kelli Sharp, Amin Boroujerdi, Oswald Steward, Z. David Luo
    Pages 195-203
  11. Marie Pertin, Romain-Daniel Gosselin, Isabelle Decosterd
    Pages 205-212
  12. Karin N. Westlund, Louis Vera-Portocarrero
    Pages 223-238
  13. Cheryl L. Marker, James D. Pomonis
    Pages 239-248
  14. Christina A. Christianson, Maripat Corr, Tony L. Yaksh, Camilla I. Svensson
    Pages 249-260
  15. Ruixin Zhang, Lixing Lao
    Pages 261-273
  16. Back Matter
    Pages 285-287

About this book


The advancements of medical technology, improvements in medical care, and increased patients’ life span make pain research and related drug development high priorities for both the research community and pharmaceutical companies. Rapid development of basic science research tools, such as techniques of flurometric labeling, genomic and proteomic high throughput screening, and genetically modified animals, promotes the swift acceleration of pain research to a stage allowing integrated investigations of pain processing mechanisms at the single cell and/or molecule level, and in a spatially and temporally controlled manner.  Using multidisciplinary approaches, we can dissect the complicity of the sensory circuits connecting peripheral stimulation to maladaptive changes in the sensory pathways as well as pain perceptions at the central nervous system. Pain Research: Methods and Protocols, Second Edition provides advanced techniques and animal models that are critical for integrated pain research. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Pain Research: Methods and Protocols, Second Edition serves as an ideal guide to novice pain researchers who may not have extensive experiences in the field, or to experienced pain researchers who would like to expand their research in new directions and/or to new mechanisms in different models. 


clinical pain research flurometric labeling high throughput screening rat pain model synaptic biomarkers translational pain research

Editors and affiliations

  • Z. David Luo
    • 1
  1. 1.Irvine Medical Center, Dept. Anesthesiology & PharmacologyUniversity of CaliforniaOrangeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-560-2
  • Online ISBN 978-1-61779-561-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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