Transgenic Plants

Methods and Protocols

  • Jim M. Dunwell
  • Andy C. Wetten

Part of the Methods in Molecular Biology book series (MIMB, volume 847)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Selection and Detection Methods

  3. Transformation Technology: Lower Plants

    1. Front Matter
      Pages 33-33
    2. Juliane Neupert, Ning Shao, Yinghong Lu, Ralph Bock
      Pages 35-47
  4. Transformation Technology: Rice

    1. Front Matter
      Pages 49-49
    2. Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui
      Pages 75-90
    3. Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, Kiichi Fukui
      Pages 91-106
  5. Transformation Technology: Cereals and Other Monocots

    1. Front Matter
      Pages 107-107
    2. Songul Gurel, Ekrem Gurel, Tamara I. Miller, Peggy G. Lemaux
      Pages 109-122
    3. Mario Gils, Myroslava Rubtsova, Katja Kempe
      Pages 123-135
    4. Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange, Preben Bach Holm
      Pages 151-161
    5. Ghulam Kadir Ahmad Parveez, Bohari Bahariah
      Pages 163-175
    6. Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, Mat Yunus Abdul Masani
      Pages 177-188
  6. Transformation Technology: Dicots

    1. Front Matter
      Pages 189-189
    2. César Petri, Ralph Scorza, Chinnathambi Srinivasan
      Pages 191-199
    3. Manjul Dutt, Zhijian T. Li, Sadanand A. Dhekney, Dennis J. Gray
      Pages 201-213
    4. Sadanand A. Dhekney, Zhijian T. Li, Manjul Dutt, Dennis J. Gray
      Pages 215-225
    5. Tianzhen Zhang, Tianzhi Chen
      Pages 237-243
    6. Andy C. Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi
      Pages 255-265
    7. Ryutaro Aida
      Pages 267-274
    8. Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi et al.
      Pages 275-289
  7. Gene Targeting, Silencing and Directed Mutation

    1. Front Matter
      Pages 291-291
    2. Isam Fattash, Basel Khraiwesh, M. Asif Arif, Wolfgang Frank
      Pages 293-315
    3. Annelies De Paepe, Sylvie De Buck, Jonah Nolf, Ann Depicker
      Pages 317-333
    4. Leandro H. Estornell, Antonio Granell, Diego Orzaez
      Pages 351-368
    5. Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, Atsushi Komamine
      Pages 379-390
    6. L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, Spencer Jones
      Pages 399-416
  8. Metabolic Engineering and Pharming

    1. Front Matter
      Pages 417-417
    2. Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-López, Sol M. Rivera et al.
      Pages 419-435
    3. Flavio Henrique-Silva, Andrea Soares-Costa
      Pages 437-450
  9. Field Tests

    1. Front Matter
      Pages 481-481
    2. Hanna Johansson Jänkänpää, Stefan Jansson
      Pages 483-494
  10. Back Matter
    Pages 495-497

About this book


In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants.  Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.


Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.


Transgenic Plants algae crop species dicots modified metabolism monocots pharmaceuticals plant gene rice transgenic Arabidopsis

Editors and affiliations

  • Jim M. Dunwell
    • 1
  • Andy C. Wetten
    • 2
  1. 1.School of Biological SciencesUniversity of ReadingReading, BerkshireUnited Kingdom
  2. 2.Dept. of Agricultural Botany, School of Biological SciencesUniversity of ReadingReadingUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-557-2
  • Online ISBN 978-1-61779-558-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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