Host-Fungus Interactions

Methods and Protocols

  • Alexandra C. Brand
  • Donna M. MacCallum

Part of the Methods in Molecular Biology book series (MIMB, volume 845)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Gene Disruption

  3. Modulation of Gene Expression: RNAi Gene Knockdown

    1. Front Matter
      Pages 149-149
    2. Michael L. Skowyra, Tamara L. Doering
      Pages 165-186
    3. João F. Menino, Agostinho J. Almeida, Fernando Rodrigues
      Pages 187-198
  4. Modulation of Gene Expression: Regulatable Promoters

    1. Front Matter
      Pages 199-199
    2. Michael Weyler, Joachim Morschhäuser
      Pages 201-210
    3. Lorina G. Baker, Jennifer K. Lodge
      Pages 211-226
    4. Vitor Cabral, Murielle Chauvel, Arnaud Firon, Mélanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi et al.
      Pages 227-244
  5. Host Responses to Infection In Vitro

    1. Front Matter
      Pages 245-245
    2. Héctor M. Mora-Montes, Christopher McKenzie, Judith M. Bain, Leanne E. Lewis, Lars P. Erwig, Neil A. R. Gow
      Pages 247-260
    3. Joanne Gibson, Neil A. R. Gow, Simon Y. C. Wong
      Pages 261-275
    4. Alieke G. Vonk, Mihai G. Netea, Bart Jan Kullberg
      Pages 277-287
    5. Torsten Wöllert, Christiane Rollenhagen, George M. Langford, Paula Sundstrom
      Pages 289-302
    6. Ann M. Kerrigan, Maria da Glória Teixeira de Sousa, Gordon D. Brown
      Pages 303-317
    7. Annemiek B. van Spriel, Alessandra Cambi
      Pages 319-331
    8. Jeanette Wagener, Daniela Mailänder-Sanchez, Martin Schaller
      Pages 333-344
    9. Lea Gregson, William W. Hope, Susan J. Howard
      Pages 361-367
    10. Marta Riera, Emilia Moreno-Ruiz, Sophie Goyard, Christophe d’Enfert, Guilhem Janbon
      Pages 369-377
  6. Fungal Responses During Infection

    1. Front Matter
      Pages 379-379
    2. Alexandre Melo Bailão, Maristela Pereira, Silvia Maria Salem-Izacc, Clayton Luiz Borges, Célia Maria de Almeida Soares
      Pages 381-396
    3. Caroline Westwater, David A. Schofield
      Pages 397-409
    4. Anja Lüttich, Sascha Brunke, Bernhard Hube
      Pages 411-421
  7. Host Responses to Infection In Vivo

    1. Front Matter
      Pages 423-423
    2. Luis Castillo, Donna M. MacCallum
      Pages 425-434
    3. Zaneeta Dhesi, Susanne Herbst, Darius Armstrong-James
      Pages 435-444
  8. Non-mammalian Model Systems for Host–Fungal Interactions

    1. Front Matter
      Pages 445-445
    2. Maged Muhammed, Jeffrey J. Coleman, Eleftherios Mylonakis
      Pages 447-454
    3. Michail S. Lionakis, Dimitrios P. Kontoyiannis
      Pages 455-468
    4. John Fallon, Judy Kelly, Kevin Kavanagh
      Pages 469-485
    5. Ilse D. Jacobsen, Katharina Große, Bernhard Hube
      Pages 487-496
  9. Mammalian Hosts as Infection Models

    1. Front Matter
      Pages 497-497
    2. Donna M. MacCallum
      Pages 499-509
    3. Guillaume Desoubeaux, Jacques Chandenier
      Pages 511-518
    4. A. George Smulian
      Pages 519-525
    5. Durdana Rahman, Mukesh Mistry, Selvam Thavaraj, Julian R. Naglik, Stephen J. Challacombe
      Pages 527-535
    6. Donatella Pietrella, Brice Enjalbert, Ute Zeidler, Sadri Znaidi, Anna Rachini, Anna Vecchiarelli et al.
      Pages 537-546
    7. Jeniel E. Nett, Karen Marchillo, David R. Andes
      Pages 547-556
    8. Karl V. Clemons, David A. Stevens
      Pages 557-567

About this book


Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.  Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.


Fungal diseases Fungal infection models Genetic manipulation Host-fungus interactions Model systems Pathogenic fungi

Editors and affiliations

  • Alexandra C. Brand
    • 1
  • Donna M. MacCallum
    • 2
  1. 1.Institute of Medical Sciences, School of Medical SciencesUniversity of AberdeenAberdeenUnited Kingdom
  2. 2.Institute of Medical Sciences, School of Medical SciencesUniversity of AberdeenAberdeenUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-538-1
  • Online ISBN 978-1-61779-539-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Chemical Manufacturing
Health & Hospitals
Consumer Packaged Goods