Methods and Protocols

  • Michael A. McGuckin
  • David J. Thornton

Part of the Methods in Molecular Biology book series (MIMB, volume 842)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Julia R. Davies, Claes Wickström, David J. Thornton
    Pages 27-47
  3. Ceri A. Harrop, David J. Thornton, Michael A. McGuckin
    Pages 49-66
  4. Mehmet Kesimer, John K. Sheehan
    Pages 67-79
  5. Malin E. V. Johansson, Gunnar C. Hansson
    Pages 109-121
  6. Franz-Georg Hanisch, Carol L. Kinlough, Simon Staubach, Rebecca P. Hughey
    Pages 123-140
  7. Catherine A. Hayes, Szilard Nemes, Samah Issa, Chunsheng Jin, Niclas G. Karlsson
    Pages 141-163
  8. Stephen D. Carrington, Jane A. Irwin, Li Liu, Pauline M. Rudd, Elizabeth Matthews, Anthony P. Corfield
    Pages 191-215
  9. Lena Holm, Mia Phillipson
    Pages 217-227
  10. Jenny K. Gustafsson, Henrik Sjövall, Gunnar C. Hansson
    Pages 237-243
  11. Lubna H. Abdullah, Cédric Wolber, Mehmet Kesimer, John K. Sheehan, C. William Davis
    Pages 259-277
  12. Lucia Piccotti, Burton F. Dickey, Christopher M. Evans
    Pages 279-295
  13. Yong H. Sheng, Sumaira Z. Hasnain, Chin Wen Png, Michael A. McGuckin, Sara K. Lindén
    Pages 297-312
  14. Back Matter
    Pages 327-329

About this book


Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells.  In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques  for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.


Mucin Biology biochemical analysis human bronchial epithelial in situ in vivo measurement intracellular trafficking isolation mucin core proteins mucus thickness physiology and pathophysiology

Editors and affiliations

  • Michael A. McGuckin
    • 1
  • David J. Thornton
    • 2
  1. 1.Mucosal Diseases ProgramMater Medical Research InstituteSouth BrisbaneAustralia
  2. 2.Fac. Life Sciences, Wellcome Trust Centre forUniversity of ManchesterManchesterUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-512-1
  • Online ISBN 978-1-61779-513-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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