Methods and Protocols

  • Françoise Rédini

Part of the Methods in Molecular Biology book series (MIMB, volume 836)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Proteoglycans

    1. Front Matter
      Pages 1-1
    2. Mauricio Cortes, James R. Mensch, Miriam Domowicz, Nancy B. Schwartz
      Pages 3-21
    3. Maziar Rahmani, Jon M. Carthy, Bruce M. McManus
      Pages 35-52
    4. Norihiko Sasaki, Shoko Nishihara
      Pages 53-61
  3. Glycosaminoglycans Analysis

    1. Front Matter
      Pages 97-97
    2. Nathalie Mainreck, Stéphane Brézillon, Ganesh D. Sockalingum, François-Xavier Maquart, Michel Manfait, Yanusz Wegrowski
      Pages 117-130
    3. Derek J. Langeslay, Christopher J. Jones, Szabolcs Beni, Cynthia K. Larive
      Pages 131-144
    4. Katarzyna A. Uniewicz, Alessandro Ori, Timothy R. Rudd, Marco Guerrini, Mark C. Wilkinson, David G. Fernig et al.
      Pages 171-182
    5. Elisabeth Miot-Noirault, Aurélien Vidal, Philippe Auzeloux, Caroline Peyrode, Jean-Claude Madelmont, Jean-Michel Chezal
      Pages 183-198
  4. PGs: Multifunctional Cell Regulators

    1. Front Matter
      Pages 199-199
    2. Elin Rönnberg, Gunnar Pejler
      Pages 201-217
  5. Proteoglycans Involvement in Pathophysiology

    1. Front Matter
      Pages 257-257
    2. Chia-Yang Liu, Winston Whei-Yang Kao
      Pages 285-290
    3. Eon Jeong Nam, Pyong Woo Park
      Pages 291-305
    4. Steven Georges, Dominique Heymann, Marc Padrines
      Pages 307-322
    5. Marc Baud’Huin, Céline Charrier, Gwenola Bougras, Régis Brion, Frédéric Lezot, Marc Padrines et al.
      Pages 323-337
    6. Mohamed Ouzzine, Narayanan Venkatesan, Sylvie Fournel-Gigleux
      Pages 339-355
  6. Back Matter
    Pages 357-359

About this book


Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research.  In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels.  The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans.  The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study.


Aggrecan Cell regulators GAG chain structure Glycosaminoglycans Heparin sulphate proteoglycans Pathophysiology Proteins and saccharides Serglycin Therapeutic value

Editors and affiliations

  • Françoise Rédini
    • 1
  1. 1., Faculté de MédicineINSERM U957 - EA 3822NantesFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-497-1
  • Online ISBN 978-1-61779-498-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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