Microbial Metabolic Engineering

Methods and Protocols

  • Qiong Cheng

Part of the Methods in Molecular Biology book series (MIMB, volume 834)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Julia Jüergensen, Nele Ilmberger, Wolfgang R. Streit
    Pages 1-16
  3. Sabine Bastian, Frances H. Arnold
    Pages 17-31
  4. Samuel Million-Weaver, David L. Alexander, Jennifer M. Allen, Manel Camps
    Pages 33-48
  5. Xiquan Liang, Lansha Peng, Billyana Tsvetanova, Ke Li, Jian-Ping Yang, Tony Ho et al.
    Pages 93-109
  6. Shaoming Mao, Kaizhi Jia, Yanping Zhang, Yin Li
    Pages 137-151
  7. Qiong Cheng, Kristin Ruebling-Jass, Jianzhong Zhang, Qi Chen, Kevin M. Croker
    Pages 177-196
  8. Kathleen A. Curran, Nathan C. Crook, Hal S. Alper
    Pages 197-216
  9. Ying Zha, Ronald Slomp, Johan van Groenestijn, Peter J. Punt
    Pages 245-259
  10. Yong Tao, Qiong Cheng, Alexander D. Kopatsis
    Pages 283-303
  11. Back Matter
    Pages 317-319

About this book


Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. In Microbial Metabolic Engineering: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study metabolic engineering. These include methods and techniques to engineer genes and pathways, use of modern biotechnology tools in microbial metabolic engineering, and examples of metabolic engineering for real world applications such as whole cell biosensors and acetate control in large scale fermentation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Microbial Metabolic Engineering: Methods and Protocols seeks to provide researchers with an overview of key topics on microbial metabolic engineering.


FACS analysis Metabolic Engineering Microbial bacteria biomass hydrolysates cell biosensors engineer genes fermentation engineers microfermentors omics tools yeasts

Editors and affiliations

  • Qiong Cheng
    • 1
  1. 1.Central Research & DevelopmentE.I. Du Pont de Nemours and CompanyWilmingtonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-482-7
  • Online ISBN 978-1-61779-483-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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