Ubiquitin Family Modifiers and the Proteasome

Reviews and Protocols

  • R. Jürgen Dohmen
  • Martin Scheffner

Part of the Methods in Molecular Biology book series (MIMB, volume 832)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Enzymology and Substrates of Ubiquitin Family Modifiers

    1. Front Matter
      Pages 13-13
    2. A. Maxwell Burroughs, Lakshminarayan M. Iyer, L. Aravind
      Pages 15-63
    3. Marion Schnellhardt, Kristina Uzunova, Veronika N. Bade, Anke Krause, Stefan R. Weisshaar, Gerrit J. K. Praefcke et al.
      Pages 81-92
    4. Annette Flotho, Andreas Werner, Tobias Winter, Andrea S. Frank, Heidi Ehret, Frauke Melchior
      Pages 93-110
    5. Kirstin Keusekotten, Gerrit J. K. Praefcke
      Pages 111-123
    6. Annette Aichem, Marcus Groettrup
      Pages 125-132
    7. Orsolya Leidecker, Dimitris P. Xirodimas
      Pages 133-140
    8. Larissa A. Durfee, Jon M. Huibregtse
      Pages 141-149
    9. Kristin E. Burns, K. Heran Darwin
      Pages 151-160
  3. Recognition and Chain Formation of UbF Modifiers

    1. Front Matter
      Pages 161-161
    2. Sjoerd J. L. van Wijk, Magda Bienko, Ivan Dikic
      Pages 163-171
    3. Fabienne Aillet, Fernando Lopitz-Otsoa, Roland Hjerpe, Mónica Torres-Ramos, Valérie Lang, Manuel S. Rodríguez
      Pages 173-183
    4. Kim Newton, Marissa L. Matsumoto, Ronald E. Ferrando, Katherine E. Wickliffe, Michael Rape, Robert F. Kelley et al.
      Pages 185-196
    5. Anja Bremm, David Komander
      Pages 219-228
    6. Ivan Matic, Ronald T. Hay
      Pages 239-247
    7. Julian Stingele, Uwe W. Roder, Shahri Raasi
      Pages 263-277
    8. Xiang Chen, Kylie J. Walters
      Pages 279-303
  4. Proteasome Biogenesis, Regulation, and Function

  5. Protein Quality Control

    1. Front Matter
      Pages 453-453
    2. Scott A. Houck, Sangita Singh, Douglas M. Cyr
      Pages 455-461
    3. Mark S. Hipp, Kirill Bersuker, Ron R. Kopito
      Pages 463-472
    4. Michael Dreiseidler, Niko Dick, Jörg Höhfeld
      Pages 473-487
    5. Joseph R. Tran, Jeffrey L. Brodsky
      Pages 505-518
    6. Alexandra Segref, Thorsten Hoppe
      Pages 531-544
  6. Cellular and Chemical Strategies to Study and Manipulate UbF-Related Processes

    1. Front Matter
      Pages 545-545
    2. Petra Hänzelmann, Antje Schäfer, Daniel Völler, Hermann Schindelin
      Pages 547-576
    3. Frank J. Bruzzese, Michael A. Milhollen, James M. Gavin, Helen R. Josephine, James E. Brownell
      Pages 577-588
    4. Silvia Eger, Martin Scheffner, Andreas Marx, Marina Rubini
      Pages 589-596
    5. Farid El Oualid, Dharjath S. Hameed, Dris El Atmioui, Henk Hilkmann, Huib Ovaa
      Pages 597-609
    6. Christof Taxis, Michael Knop
      Pages 611-626
    7. Kimberly Cornish Carmony, Kyung-Bo Kim
      Pages 627-638

About this book


The ubiquitin-proteasome system (UPS) and ubiquitin-related modifiers are not only involved in cellular protein quality control but also in the regulation of many fundamental cellular processes/pathways as well as in their disease-relevant aberrations. Ubiquitin Family Modifiers and Proteasome: Reviews and Protocols presents both novel developments in UPS research and important methods related to the main recent advances in the field of ubiquitin family modifiers. Divided into five convenient sections, this volume focuses on the enzymology and substrate identification of ubiquitin family modifiers, the recognition and chain formation of these modifiers, the analysis of proteasome biogenesis and function, protein quality control, and finally the use of small molecules and strategies to study or manipulate the function of the UPS and of ubiquitin family modifiers, respectively. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Ubiquitin Family Modifiers and Proteasome: Reviews and Protocols will be of great use to investigators and students engaged in both basic and applied research in life sciences.



UbF modifiers Ubiquitin-Proteasome system function Ubiquitin-related modifiers cellular ubiquitylation tissue-specific ubiquitylation

Editors and affiliations

  • R. Jürgen Dohmen
    • 1
  • Martin Scheffner
    • 2
  1. 1.Institut für GenetikUniversität KölnKölnGermany
  2. 2.Fak. BiologieUniversität KonstanzKonstanzGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-473-5
  • Online ISBN 978-1-61779-474-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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