Liver Stem Cells

Methods and Protocols

  • Takahiro Ochiya

Part of the Methods in Molecular Biology book series (MIMB, volume 826)

Table of contents

  1. Front Matter
    Pages i-x
  2. Several Hepatic Progenitor Cells

  3. Hepatic Differentiation from Stem Cells

    1. Front Matter
      Pages 59-59
    2. Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, Suradej Hongeng
      Pages 73-87
    3. Tetsuya Ishikawa, Keitaro Hagiwara, Takahiro Ochiya
      Pages 103-114
    4. Kenji Kawabata, Mitsuru Inamura, Hiroyuki Mizuguchi
      Pages 115-124
    5. Laura Gomez-Santos, Mercedes Vazquez-Chantada, Jose Maria Mato, Maria Luz Martinez-Chantar
      Pages 133-149
  4. BD Formation from Stem Cells

  5. Liver Stem Cells and Hepatocarcinogenesis

    1. Front Matter
      Pages 161-161
  6. Application of Liver Stem Cells for Cell Therapy

    1. Front Matter
      Pages 177-177
    2. Moubarak Mouiseddine, Sabine François, Maâmar Souidi, Alain Chapel
      Pages 179-188
    3. Yasuyuki Sakai, M. Nishikawa, F. Evenou, M. Hamon, H. Huang, K. P. Montagne et al.
      Pages 189-216
  7. Back Matter
    Pages 225-226

About this book


Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stem cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells: Methods and Protocols, expert researchers focus on  several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and  application of liver stem cells for cell therapy.  These topics shed light on stem cell technology which may lead to the development of effective clinical modalities for human liver diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Thorough and intuitive, Liver Stem Cells: Methods and Protocols seeks to aid scientists in the further study of preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries.


Bile ductal cell Stem Cells Stem cell transplantation cell therapy hepatic progenitor cells liver injuries

Editors and affiliations

  • Takahiro Ochiya
    • 1
  1. 1.Research Inst.National Cancer CenterTokyoJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-467-4
  • Online ISBN 978-1-61779-468-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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