Methods and Protocols

  • Richard Milner

Part of the Methods in Molecular Biology book series (MIMB, volume 814)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Overview of Astrocyte Biology

    1. Front Matter
      Pages 1-1
    2. Bruce R. Ransom, Christopher B. Ransom
      Pages 3-7
    3. Nancy Ann Oberheim, Steven A. Goldman, Maiken Nedergaard
      Pages 23-45
  3. Isolation and Culture of Astrocytes

    1. Front Matter
      Pages 47-47
    2. Jean de Vellis, Ruth Cole
      Pages 49-59
    3. Tracy F. Uliasz, Mary E. Hamby, Nicole A. Jackman, James A. Hewett, Sandra J. Hewett
      Pages 61-79
    4. Jennifer V. Welser, Richard Milner
      Pages 81-91
    5. Amber E. Kerstetter, Robert H. Miller
      Pages 93-104
    6. Thomas J. Lukas, Ai Ling Wang
      Pages 105-115
    7. Cristina Ballarin, Antonella Peruffo
      Pages 117-126
    8. Dennis J. Bobilya
      Pages 127-135
    9. Ariane Sharif, Vincent Prevot
      Pages 137-151
  4. Analysis of Astrocyte Functions In Vitro

  5. Co-culture Systems to Analyse Astrocyte Interactions with Other Cell Types

    1. Front Matter
      Pages 325-325
    2. Panpan Yu, Hang Wang, Yasuhiro Katagiri, Herbert M. Geller
      Pages 327-340
    3. Fardad T. Afshari, James W. Fawcett
      Pages 381-391
    4. N. Joan Abbott, Diana E. M. Dolman, Svetlana Drndarski, Sarah M. Fredriksson
      Pages 415-430
    5. Kaushik K. Shah, Li Yang, Thomas J. Abbruscato
      Pages 431-449
    6. Li Yang, Kaushik K. Shah, Thomas J. Abbruscato
      Pages 451-466
  6. Analysis of Astrocyte Functions In Vivo

  7. Back Matter
    Pages 571-575

About this book


Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma.  Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Key cellular, molecular and biochemical techniques are used to study the many and varied functions of this fascinating cell.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.




Authoritative and practical Astrocytes: Methods and Protocols seeks to aid scientists and researchers looking to use more specialized techniques to examine astrocyte function. 


CNS adjusting blood flow dynamics astrocytes blood-brain barrier buffering K+ ions glutamate and NH4 detoxification mediate neurovascular coupling multifunctional versatile cells neuronal architecture regulation of extracellular pH

Editors and affiliations

  • Richard Milner
    • 1
  1. 1.Dept. Molecular & ExperimentalScripps Research InstituteLa JollaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-451-3
  • Online ISBN 978-1-61779-452-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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