SELDI-TOF Mass Spectrometry

Methods and Protocols

  • Charlotte H. Clarke
  • Diane L. Bankert McCarthy

Part of the Methods in Molecular Biology book series (MIMB, volume 818)

Table of contents

  1. Front Matter
    Pages i-x
  2. Lee Lomas, Charlotte H. Clarke, Vanitha Thulasiraman, Eric Fung
    Pages 1-10
  3. Luc Guerrier, Frederic Fortis, Egisto Boschetti
    Pages 11-33
  4. Enrique A. Dalmasso, Dominic Caseñas
    Pages 35-48
  5. Yiting Li, Tu Anh Dang, Tsz-Kwong Man
    Pages 81-96
  6. Ronald L. Woodbury, Diane L. Bankert McCarthy, Amanda L. Bulman
    Pages 97-107
  7. Anja H. Simonsen
    Pages 109-117
  8. Sean R. Downing, Giannoula L. Klement
    Pages 153-170
  9. Lin Ji, Gitanjali Jayachandran, Jack A. Roth
    Pages 199-216
  10. Eddie Takahashi, Anita Howe, Ole Vesterqvist, Zhaosheng Lin
    Pages 227-236
  11. Back Matter
    Pages 237-239

About this book


SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characterization in diverse applications including diagnostics, drug development, and basic research. SELDI-based biomarker studies can typically be divided into four phases: discovery, validation, purification and identification, and assay development. SELDI-TOF Mass Spectrometry: Methods and Protocols provides an overview of the current applications of SELDI-TOF MS (surface enhanced laser desorption/ionization time-of-flight mass spectrometry), with an emphasis on study and experimental design, data analysis and interpretation, and assay development. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, SELDI-TOF Mass Spectrometry: Methods and Protocols will provide information on optimizing study design, experimental protocols, and data analysis and interpretation to yield robust biomarkers and biomarker assays, using examples from different disease areas.


Candidate biomarkers ProteinChip technology biomarker assay chromatography protein profiling surface enhanced laser desorption/ionization time-of-flight mass spectrometry

Editors and affiliations

  • Charlotte H. Clarke
    • 1
  • Diane L. Bankert McCarthy
    • 2
  1. 1.The University of Texas, Department of Translational ResearchM.D. Anderson Cancer CenterHoustonUSA
  2. 2.Bio-Rad LaboratoriesMalvernUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-417-9
  • Online ISBN 978-1-61779-418-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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