Chemical Proteomics

Methods and Protocols

  • Gerard Drewes
  • Marcus Bantscheff

Part of the Methods in Molecular Biology book series (MIMB, volume 803)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Gerard Drewes
      Pages 15-21
  3. Small Molecules and Probe Design

    1. Front Matter
      Pages 23-23
    2. Uwe Rix, Manuela Gridling, Giulio Superti-Furga
      Pages 25-38
    3. Akira Kawamura, Doina M. Mihai
      Pages 65-75
    4. Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, Daniel C. Liebler
      Pages 77-95
  4. Target Discovery and Target Validation

    1. Front Matter
      Pages 127-127
    2. Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, Steven A. Carr
      Pages 129-140
    3. Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, Marcus Bantscheff
      Pages 141-155
    4. Xia Ge, Daniel S. Sem
      Pages 157-165
    5. Duangnapa Kovanich, Thin Thin Aye, Albert J. R. Heck, Arjen Scholten
      Pages 167-181
    6. Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, Celia R. Berkers
      Pages 183-204
  5. Ligand Discovery

    1. Front Matter
      Pages 247-247
    2. Dominick E. Casalena, Dina Wassaf, Angela N. Koehler
      Pages 249-263
    3. Andrea Wolf, Satoko Shimamura, Friedrich B. M. Reinhard
      Pages 265-271
    4. Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, Christoph Steinbeck
      Pages 273-296
  6. Back Matter
    Pages 309-313

About this book


The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function.  In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.


Mass Spectrometry amino acids in cell culture (SILAC) based chemical probes isotope labeling molecule ligand molecules protein function quantitative immunodetection

Editors and affiliations

  • Gerard Drewes
    • 1
  • Marcus Bantscheff
    • 2
  1. 1.CellZome AGHeidelbergGermany
  2. 2.CellZome AGHeidelbergGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-363-9
  • Online ISBN 978-1-61779-364-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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