Protein Expression in Mammalian Cells

Methods and Protocols

  • James L Hartley

Part of the Methods in Molecular Biology book series (MIMB, volume 801)

Table of contents

  1. Front Matter
    Pages i-xi
  2. James L. Hartley
    Pages 1-12
  3. Lucia Baldi, David L. Hacker, Carine Meerschman, Florian M. Wurm
    Pages 13-26
  4. Barbara Ann Jardin, Cynthia B. Elias, Satya Prakash
    Pages 41-63
  5. Oliver Gresch, Ludger Altrogge
    Pages 65-74
  6. Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, Sabine Spiegl-Kreinecker, Walter Berger, Michael Grusch
    Pages 75-92
  7. Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, Nicolas Mermod
    Pages 93-110
  8. Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, Tina M. Sauerwald
    Pages 111-123
  9. Mariati, Steven C. L. Ho, Miranda G. S. Yap, Yuansheng Yang
    Pages 125-135
  10. Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, Raymond J. Owens
    Pages 137-159
  11. Zahra Assur, Wayne A. Hendrickson, Filippo Mancia
    Pages 173-187
  12. Renate Kunert, Karola Vorauer-Uhl
    Pages 213-226
  13. Ralph F. Hopkins, Vanessa E. Wall, Dominic Esposito
    Pages 251-268
  14. Back Matter
    Pages 269-276

About this book


Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease.  In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.


Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.


Cell biology Cellular manipulation Mammalian models Protein organelles Recombinant proteins

Editors and affiliations

  • James L Hartley
    • 1
  1. 1.SAIC-Frederick, Inc., Protein Expression LaboratoryNational Cancer Institute, FrederickFrederickUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-351-6
  • Online ISBN 978-1-61779-352-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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