Methods and Protocols

  • Joseph X. DiMario

Part of the Methods in Molecular Biology book series (MIMB, volume 798)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Isolation and Analysis of Skeletal Muscle Precursor Cells

    1. Front Matter
      Pages 1-1
    2. Ariya D. Lapan, Emanuela Gussoni
      Pages 3-19
    3. Alessandra Pasut, Paul Oleynik, Michael A. Rudnicki
      Pages 53-64
    4. Mattia Quattrocelli, Giacomo Palazzolo, Ilaria Perini, Stefania Crippa, Marco Cassano, Maurilio Sampaolesi
      Pages 65-76
    5. Eric J. Cavanaugh, Jennifer R. Crew, Joseph X. DiMario
      Pages 77-84
  3. Non-Mammalian Models of Myogenesis

    1. Front Matter
      Pages 125-125
    2. Ginny R. Morriss, Anton L. Bryantsev, Maria Chechenova, Elisa M. LaBeau, TyAnna L. Lovato, Kathryn M. Ryan et al.
      Pages 127-152
    3. Nathan C. Bird, Stefanie E. Windner, Stephen H. Devoto
      Pages 153-169
    4. Kristy J. Wilson, Hiroshi Qadota, Guy M. Benian
      Pages 171-181
  4. Experimental Models and Analysis of Skeletal Muscle Exercise and Disuse

  5. Generation of Viral Vectors and Transgenic Mice

    1. Front Matter
      Pages 265-265
    2. Jin-Hong Shin, Yongping Yue, Dongsheng Duan
      Pages 267-284
    3. Christophe Pichavant, Jacques P. Tremblay
      Pages 285-295
  6. Muscle Profiling

    1. Front Matter
      Pages 309-309
    2. Shephali Bhatnagar, Siva K. Panguluri, Ashok Kumar
      Pages 311-324
    3. Vassilis Mougios, Anatoli Petridou
      Pages 325-355
    4. Caroline Lewis, Philip Doran, Kay Ohlendieck
      Pages 357-369
  7. Experimental Approaches in Calcium Imaging of Skeletal Muscle

    1. Front Matter
      Pages 371-371
    2. Jerry P. Eu, Gerhard Meissner
      Pages 373-382
    3. Mariana Casas, Francisco Altamirano, Enrique Jaimovich
      Pages 383-393
    4. Noah Weisleder, Jingsong Zhou, Jianjie Ma
      Pages 395-410
  8. Analysis of Gene Promoter Transcriptional Activity

    1. Front Matter
      Pages 423-423
    2. Charis L. Himeda, Phillip W. L. Tai, Stephen D. Hauschka
      Pages 425-443
    3. Phillip W. L. Tai, Catherine L. Smith, John C. Angello, Stephen D. Hauschka
      Pages 445-459
    4. Sarah M. Senf, Andrew R. Judge
      Pages 461-472
  9. RNA-Mediated Gene Regulation

    1. Front Matter
      Pages 473-473
    2. Zhan-Peng Huang, Ramón Espinoza-Lewis, Da-Zhi Wang
      Pages 475-490
    3. Muriel Golzio, Jean-Michel Escoffre, Justin Teissié
      Pages 491-501
  10. Analysis of Protein-DNA Interactions

    1. Front Matter
      Pages 503-503
    2. Yasuyuki Ohkawa, Chandrashekara Mallappa, Caroline S. Dacwag Vallaster, Anthony N. Imbalzano
      Pages 517-530
    3. Yasuyuki Ohkawa, Chandrashekara Mallappa, Caroline S. Dacwag Vallaster, Anthony N. Imbalzano
      Pages 531-542
  11. Back Matter
    Pages 555-558

About this book


Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology.  Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells.  Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume.  Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and authoritative, Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.


Adaptive responses Calcium imaging Model systems Protein-DNA interactions Regenerative medicine Skeletal muscle development Skeletal muscle stem cells Stem cell isolation Viral vector delivery systems

Editors and affiliations

  • Joseph X. DiMario
    • 1
  1. 1.Chicago Medical School, Dept. Cell Biology & AnatomyRosalind Franklin Univ of Med & SciNorth ChicagoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-342-4
  • Online ISBN 978-1-61779-343-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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