Oncolytic Viruses

Methods and Protocols

  • David H. Kirn
  • Ta-Chiang Liu
  • Stephen H. Thorne

Part of the Methods in Molecular Biology book series (MIMB, volume 797)

Table of contents

  1. Front Matter
    Pages i-x
  2. Pankaj K. Agarwalla, Manish K. Aghi
    Pages 1-19
  3. Konstantin Doronin, Dmitry M. Shayakhmetov
    Pages 35-52
  4. Debanjan Dhar, Karoly Toth, William S. M. Wold
    Pages 53-63
  5. Michael A. Barry, Shannon May, Eric A. Weaver
    Pages 79-87
  6. Lucile Tran, Nadège Dartial, Cecilia Hindorf, Georges Vassaux
    Pages 89-96
  7. Atique U. Ahmed, Ilya V. Ulasov, Ronald W. Mercer, Maciej S. Lesniak
    Pages 97-109
  8. Marta M. Alonso, Hong Jiang, Candelaria Gomez-Manzano, Juan Fueyo
    Pages 111-125
  9. Jean-Simon Diallo, Markus Vähä-Koskela, Fabrice Le Boeuf, John Bell
    Pages 127-140
  10. Pavlos Msaouel, Ianko D. Iankov, Cory Allen, Stephen J. Russell, Evanthia Galanis
    Pages 141-162
  11. Steve H. Thorne
    Pages 205-215
  12. Joshua F. Heiber, Glen N. Barber
    Pages 217-238
  13. Back Matter
    Pages 239-241

About this book


Since the first report of an engineered oncolytic virus, there has been a continuing and steady increase of interest in the field, and while bench research remains vital for the translation of research in this field, its success depends on breakthroughs in clinical studies. Oncolytic Viruses: Methods and Protocols describes the construction and purification of capsid-modified adenoviruses as well as oncolytic adenoviruses, presents protocols for many individual virus species including engineering and preparation of oncolytic HSV, propagation, purification, and in vivo testing of oncolytic VSV, details properties of oncolytic reovirus and NDV, and describes the generation and testing of next generation of oncolytic vaccinia virus. As the host immune system plays a critical role in determining efficacy of oncolytic viruses, two chapters are devoted to the study of immune response. Recent advances in stem cell research have led the field in two distinct directions: the use of stem cells as carrier vehicles for oncolytic viruses and the targeting of cancer stem cells. As such, the volume describes the use of explant tissue samples from patients to potentially provide useful information predicting responses prior to clinical translation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Oncolytic Viruses: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential and vital field.


Newcastle disease virus Vaccinia virus capsid-modified adenovirus oncology oncolytic reovirus stem cells virology

Editors and affiliations

  • David H. Kirn
    • 1
  • Ta-Chiang Liu
    • 2
  • Stephen H. Thorne
    • 3
  1. 1.Jennerex Biotherapeutics, Inc.San FranciscoUSA
  2. 2.School of Medicine, Dept. Pathology & ImmunologyWashington UniversitySt.LouisUSA
  3. 3.Hillman Cancer CenterUniversity of PittsburghPittsburghUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-339-4
  • Online ISBN 978-1-61779-340-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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