Kinase Inhibitors

Methods and Protocols

  • Bernhard Kuster

Part of the Methods in Molecular Biology book series (MIMB, volume 795)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Doriano Fabbro, Sandra W. Cowan-Jacob, Henrik Möbitz, Georg Martiny-Baron
    Pages 1-34
  3. Byoung Dae Lee, Xiaojie Li, Ted M. Dawson, Valina L. Dawson
    Pages 45-54
  4. Elisa Ciraolo, Alessia Perino, Emilio Hirsch
    Pages 55-67
  5. Oleg Fedorov, Frank H. Niesen, Stefan Knapp
    Pages 109-118
  6. James S. Duncan, Timothy A. J. Haystead, David W. Litchfield
    Pages 119-134
  7. Jenny J. Fischer, Olivia Y. Graebner (neé Baessler), Mathias Dreger
    Pages 135-147
  8. Markus Schirle, Eugene C. Petrella, Scott M. Brittain, David Schwalb, Edmund Harrington, Ivan Cornella-Taracido et al.
    Pages 161-177
  9. Alexander V. Statsuk, Kevan M. Shokat
    Pages 179-190
  10. Oliver Pötz, Nicole Schneiderhan-Marra, Tanja Henzler, Thomas Herget, Thomas O. Joos
    Pages 191-202
  11. Back Matter
    Pages 255-256

About this book


Protein and lipid kinases are often the master regulators of cell signaling in eukaryotic systems. The human genome codes for more than 500 of these enzymes and their misregulation has been shown to be involved in the onset and progression of many diseases including cancer and inflammation. Therefore, small molecule kinase inhibitors have become important research tools for the elucidation of the many biological roles of kinases and their mechanisms of action. Kinase inhibitors thus also contribute significantly to the drug pipelines of the pharmaceutical and biotechnology industries and to the growing need to treat cancer and inflammation. In Kinase Inhibitors: Methods and Protocols, experts in kinase biology, drug discovery, and clinical research present a series of exemplary methods that can be used to address the many challenges facing scientists in the discovery and development of kinase inhibitors both for research and clinical use. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Kinase Inhibitors: Methods and Protocols aims to provide scientists with modern and relevant methods to accelerate or strengthen their research and drug discovery programs through the utilization of these vital regulators.


biochemical kinase activity assays cancer inflammation lipid kinases peptide substrates protein kinases small molecule kinase inhibitors

Editors and affiliations

  • Bernhard Kuster
    • 1
  1. 1.LS für Proteomik und BioanalytikTechnische Universität MünchenFreisingGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-336-3
  • Online ISBN 978-1-61779-337-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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