Methods and Protocols

  • Aron W. Fenton

Part of the Methods in Molecular Biology book series (MIMB, volume 796)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Monitoring Allosteric Function

    1. Front Matter
      Pages 1-1
    2. James K. Kranz, José C. Clemente
      Pages 3-17
    3. Jo M. Holt, Gary K. Ackers
      Pages 19-29
    4. Marta Martinez-Julvez, Olga Abian, Sonia Vega, Milagros Medina, Adrian Velazquez-Campoy
      Pages 53-70
    5. Harvey F. Fisher
      Pages 71-96
    6. Jai A. P. Shanata, Shawnalea J. Frazier, Henry A. Lester, Dennis A. Dougherty
      Pages 97-113
  3. Monitoring Allosteric Conformational Changes

    1. Front Matter
      Pages 115-115
    2. Tarjani M. Thaker, Ali I. Kaya, Anita M. Preininger, Heidi E. Hamm, T. M. Iverson
      Pages 133-174
    3. Aaron L. Lucius, P. Keith Veronese, Ryan P. Stafford
      Pages 175-186
    4. Gabriel A. Frank, Amnon Horovitz, Gilad Haran
      Pages 205-216
  4. Monitoring Allosteric Changes in Protein Dynamics/Subpopulation Distribution

    1. Front Matter
      Pages 233-233
    2. Donald J. Jacobs, Dennis R. Livesay, James M. Mottonen, Oleg K. Vorov, Andrei Y. Istomin, Deeptak Verma
      Pages 279-304
  5. Macromolecular and Ligand Engineering Allosteric Functions

  6. Computational Methods/Aids in the Study of Allostery

    1. Front Matter
      Pages 383-383
    2. Dennis R. Livesay, Kyle E. Kreth, Anthony A. Fodor
      Pages 385-398
    3. Dengming Ming, Michael E. Wall
      Pages 423-436
  7. Back Matter
    Pages 437-439

About this book


Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function.  In Allostery: Methods and Protocols, expert researchers in the field provide key techniques to investigate this biological phenomenon.  Focusing on heterotropic systems with some coverage of homotropic systems, this volume covers the monitoring of allosteric function, allosteric conformational changes, and allosteric changes in protein dynamics/sub-population distribution, as well as topics such as macromolecular and ligand engineering of allosteric functions and computational aids in the study of allostery. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.


Thorough and intuitive, Allostery: Methods and Protocols aids scientists in continuing to study ligand-induced, through-protein effects on protein function (ligand binding/catalysis), a phenomenon that is well recognized through the history of the life sciences and very poorly understood at the molecular level.


Allosteric regulation Computational methods Conformational changes Cooperative Heterotropic Homotropic Ligands Protein dynamics Protein structure/function Sub-population distribution

Editors and affiliations

  • Aron W. Fenton
    • 1
  1. 1., Dept. Biochemistry & Molecular BiologyUniversity of Kansas Medical CenterKansas CityUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2012
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-333-2
  • Online ISBN 978-1-61779-334-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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