Handbook of ELISPOT

Methods and Protocols

  • Alexander E. Kalyuzhny

Part of the Methods in Molecular Biology book series (MIMB, volume 792)

Table of contents

  1. Front Matter
    Pages i-x
  2. Setting and Performing ELISPOT Assay

    1. Front Matter
      Pages 1-1
    2. Paul V. Lehmann, Wenji Zhang
      Pages 3-23
    3. Sylvia Janetzki, Cedrik M. Britten
      Pages 25-36
  3. ELISPOT for Veterinary Research

    1. Front Matter
      Pages 37-37
    2. Jodi Hagen, Chris Hartnett, Jeffrey P. Houchins, Steeve Giguère, Alexander E. Kalyuzhny
      Pages 39-45
    3. Jeffrey R. Abbott, Ruiyu Pu, James K. Coleman, Janet K. Yamamoto
      Pages 47-63
    4. Zejing Wang, Rainer Storb, Stephen J. Tapscott, Stanley Riddell
      Pages 65-74
  4. Advanced ELISPOT Techniques

    1. Front Matter
      Pages 75-75
    2. Niklas Ahlborg, Bernt Axelsson
      Pages 77-85
    3. Jodi Hagen, Jeffrey P. Houchins, Alexander E. Kalyuzhny
      Pages 87-96
    4. Jodi Hagen, Jeffrey P. Houchins, Alexander E. Kalyuzhny
      Pages 97-104
    5. Jodi Hagen, Jeffrey P. Houchins, Alexander E. Kalyuzhny
      Pages 115-122
  5. Image and Data Analysis

    1. Front Matter
      Pages 123-123
    2. Marcus Dittrich, Paul V. Lehmann
      Pages 173-183
    3. Zoe Moodie, Leah Price, Sylvia Janetzki, Cedrik M. Britten
      Pages 185-196
  6. ELISPOT Assay for Vaccine Development and Diagnostics

    1. Front Matter
      Pages 197-197
    2. Benjamin J. Umlauf, Norman A. Pinsky, Inna G. Ovsyannikova, Gregory A. Poland
      Pages 199-218
    3. Marsha L. Feske, Miguel Medina, Edward A. Graviss, Dorothy E. Lewis
      Pages 229-241
  7. Back Matter
    Pages 257-261

About this book


Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research.  The second edition of Handbook of ELISPOT: Methods and Protocols, only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition.  Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool.


Animal models Cell research Diagnostics Enzyme-linked immunospot assay Image analysis Immune system cells Statistical data Vaccine development Veterinary research

Editors and affiliations

  • Alexander E. Kalyuzhny
    • 1
  1. 1.R & D Systems, Inc.MinneapolisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-324-0
  • Online ISBN 978-1-61779-325-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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