Molecular Chaperones

Methods and Protocols

  • Stuart K. Calderwood
  • Thomas L. Prince

Part of the Methods in Molecular Biology book series (MIMB, volume 787)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, Nahid F. Mivechi
    Pages 1-20
  3. Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson et al.
    Pages 21-32
  4. Natalie Wayne, Parul Mishra, Daniel N. Bolon
    Pages 33-44
  5. Mehdi Mollapour, Len Neckers
    Pages 67-74
  6. Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, Avrom J. Caplan
    Pages 75-81
  7. Heike Rampelt, Matthias P. Mayer, Bernd Bukau
    Pages 83-91
  8. Hong Yu Ren, Cam Patterson, Douglas M. Cyr, Meredith F. N. Rosser
    Pages 93-103
  9. André-Patrick Arrigo
    Pages 105-119
  10. Punit Kaur, Ganachari M. Nagaraja, Alexzander Asea
    Pages 121-136
  11. Jessica Gobbo, Caroline Gaucher-Di-Stasio, Stéphanie Weidmann, Jean Guzzo, Carmen Garrido
    Pages 137-143
  12. H. Elyse Ireland, John H. H. Williams
    Pages 145-153
  13. Nina C. Dempsey, Francesca Leoni, Christine Hoyle, John H. H. Williams
    Pages 155-164
  14. Anne-Laure Rérole, Gaëtan Jego, Carmen Garrido
    Pages 205-230
  15. Alexander E. Kabakov, Vladimir A. Kudryavtsev, Vladimir L. Gabai
    Pages 231-244
  16. Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, Alberto J. L. Macario
    Pages 245-254
  17. Desheng Weng, Stuart K. Calderwood, Jianlin Gong
    Pages 255-265
  18. Fernando D. Cuello-Carrión, Mariel A. Fanelli, Niubys Cayado-Gutiérrez, Gisela Castro, Daniel R. Ciocca
    Pages 267-275
  19. Ayesha Murshid, Jimmy Theriault, Jianlin Gong, Stuart K. Calderwood
    Pages 289-302
  20. Michelle M. Kouspou, John T. Price
    Pages 303-317
  21. Back Matter
    Pages 319-321

About this book


The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form.  In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.


HSF proteins HSP gene promoters Heat shock proteins Heat shock response Heat shock transcription factors Protein folding Protein quality control

Editors and affiliations

  • Stuart K. Calderwood
    • 1
  • Thomas L. Prince
    • 2
  1. 1.Beth Israel Deaconess Medical Center, Department of Radiation OncologyHarvard Medical SchoolBostonUSA
  2. 2.National Cancer Institute, Urologic Oncology BranchNational Institutes of HealthBethesdaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-294-6
  • Online ISBN 978-1-61779-295-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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