Human Embryonic and Induced Pluripotent Stem Cells

Lineage-Specific Differentiation Protocols

  • Kaiming Ye
  • Sha Jin

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Maintenance and Cryopreservation of ES and iPS Cells

  3. Generation of Human Induced Pluripotent Stem Cells: Viral and Nonviral Vector-Based Nuclear Reprogramming

    1. Front Matter
      Pages 91-91
    2. Alessandra Giorgetti, Nuria Montserrat, Juan Carlos Izpisua Belmonte
      Pages 93-111
    3. Naoki Nishishita, Chiemi Takenaka, Shin Kawamata
      Pages 113-131
    4. Maria V. Shutova, Ilya V. Chestkov, Alexandra N. Bogomazova, Maria A. Lagarkova, Sergey L. Kiselev
      Pages 133-149
    5. Hua Liu, Su Mi Choi, Yoon-Young Jang
      Pages 151-160
    6. Hiroe Ohnishi, Yasuaki Oda, Hajime Ohgushi
      Pages 173-190
  4. Generation of Patient-Specific iPS Cells for Clinical Application

    1. Front Matter
      Pages 229-229
    2. Katie Avery, Stuart Avery
      Pages 231-247
    3. Masashi Toyoda, Shogo Nagata, Hatsune Makino, Hidenori Akutsu, Takashi Tada, Akihiro Umezawa
      Pages 249-264
  5. Lineage-Specific Differentiation of hES and iPS Cells

    1. Front Matter
      Pages 265-265
    2. Xiaolong Lin, Hua Jiang, Zack Zhengyu Wang, Tong Chen
      Pages 267-275
    3. Shijun Hu, Preston Lavinghousez, Zongjin Li, Joseph C. Wu
      Pages 311-320
    4. Feng Ma, Yanzheng Gu, Natsumi Nishihama, Wenyu Yang, Ebihara Yasuhiro, Kohichiro Tsuji
      Pages 321-335
    5. Michio Noguchi, Masakatsu Sone, Daisuke Taura, Ken Ebihara, Kiminori Hosoda, Kazuwa Nakao
      Pages 351-358
    6. Deborah Ferrari, Guochun Gong, Robert A. Kosher, Caroline N. Dealy
      Pages 359-367
    7. Claire N. Medine, Zara Hannoun, Sebastian Greenhough, Catherine M. Payne, Judy Fletcher, David C. Hay
      Pages 369-386
    8. Su Mi Choi, Hua Liu, Yonghak Kim, Yoon-Young Jang
      Pages 387-396
    9. Lindsey Van Haute, Gert De Block, Inge Liebaers, Karen Sermon, Martine De Rycke
      Pages 405-411
  6. Directed Differentiation of hES and iPS Cells in 3D Environments

    1. Front Matter
      Pages 413-413
  7. Qualitative and Quantitative Analysis of Dynamics of hES Cell Differentiation

    1. Front Matter
      Pages 443-443
    2. Jason D. Gibson, Caroline M. Jakuba, Craig E. Nelson, Mark G. Carter
      Pages 445-463
  8. Back Matter
    Pages 481-486

About this book


Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world.  The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies.  Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls.


Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.


Cell replacement therapies Cryopreservation Embryonic stem cells Lineage-specific differentiation Nuclear reprogramming Regenerative medicine Three-dimensional scaffolds Tissue engineering Viral vectors hES and hiPS cells

Editors and affiliations

  • Kaiming Ye
    • 1
  • Sha Jin
    • 2
  1. 1., College of EngineeringUniversity of ArkansasFayettevilleUSA
  2. 2., College of EngineeringUniversity of ArkansasFayettevilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-266-3
  • Online ISBN 978-1-61779-267-0
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site
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