Single Molecule Enzymology

Methods and Protocols

  • Gregory I. Mashanov
  • Christopher Batters

Part of the Methods in Molecular Biology book series (MIMB, volume 778)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Volker Bormuth, Felix Zörgibel, Erik Schäffer, Jonathon Howard
    Pages 11-18
  3. Melinda Tonks Hoffman, Janet Sheung, Paul R. Selvin
    Pages 33-56
  4. Franck J. Fourniol, Carolyn A. Moores
    Pages 57-70
  5. Shane R. Nelson, M. Yusuf Ali, David M. Warshaw
    Pages 111-121
  6. Tatiana A. Nenasheva, Gregory I. Mashanov, Michelle Peckham, Justin E. Molloy
    Pages 123-142
  7. Toshio Yanagida, Yoshiharu Ishii, Akihiko Ishijima
    Pages 143-159
  8. Christopher P. Toseland, Martin R. Webb
    Pages 161-174
  9. Natalia Fili, Christopher P. Toseland, Mark S. Dillingham, Martin R. Webb, Justin E. Molloy
    Pages 193-214
  10. Takayuki Nishizaka, Yuh Hasimoto, Tomoko Masaike
    Pages 259-271
  11. Tassilo Hornung, James Martin, David Spetzler, Robert Ishmukhametov, Wayne D. Frasch
    Pages 273-289
  12. Natalia Fili, Christopher P. Toseland, Mark S. Dillingham, Martin R. Webb, Justin E. Molloy
    Pages E1-E1
  13. Back Matter
    Pages 291-294

About this book


The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy.  In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.


Cell signaling Data analysis Enzymes Sample preparation Single molecule measurements

Editors and affiliations

  • Gregory I. Mashanov
    • 1
  • Christopher Batters
    • 2
  1. 1., Division of Physical BiochemistryNational Institute for Medical ResearchLondonUnited Kingdom
  2. 2.Physiologisches Institute, Zelluläre PhysiologieLudwig-Maximilian Universität MünchenMunichGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-260-1
  • Online ISBN 978-1-61779-261-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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