Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 773)
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Table of contents (23 protocols)
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Epigenetic Changes Associated with Seed Dormancy
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Spectroscopic and Whole Tissue Analyses
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Front Matter
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Analyses of Reactive Oxygen Species, Protein Damage and Repair
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Front Matter
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Analyses of Reactive Oxygen Species, Protein Damage and Repair
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Bioinformatic Resources
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Front Matter
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Back Matter
About this book
Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world’s food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8’-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that willprove invaluable for both applied and fundamental seed research.
Editors and Affiliations
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Dept. Biological Sciences, Simon Fraser Unviersity, Burnaby, Canada
Allison R. Kermode
Bibliographic Information
Book Title: Seed Dormancy
Book Subtitle: Methods and Protocols
Editors: Allison R. Kermode
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-231-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-230-4Published: 19 August 2011
Softcover ISBN: 978-1-4939-6139-9Published: 23 August 2016
eBook ISBN: 978-1-61779-231-1Published: 08 September 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 423
Topics: Plant Sciences, Plant Physiology
Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma