Proprotein Convertases

  • Majambu Mbikay
  • Nabil G. Seidah

Part of the Methods in Molecular Biology book series (MIMB, volume 768)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Reminiscences

  3. Biochemistry and Cell Biology

  4. Molecular Biology and Genetics

    1. Front Matter
      Pages 189-189
    2. Haidy Tadros, Gunther Schmidt, Francine Sirois, Majambu Mbikay
      Pages 217-230
    3. Hélène Choquet, Pieter Stijnen, John W.M. Creemers
      Pages 247-253
  5. Experimental Models

    1. Front Matter
      Pages 255-255
    2. Michael G. Morash, Kelly Soanes, Younes Anini
      Pages 257-271
    3. Babykumari P. Chitramuthu, Hugh P.J. Bennett
      Pages 273-296
    4. Jonathan Wardman, Lloyd D. Fricker
      Pages 307-323
    5. Ramona M. Rodriguiz, Jennifer S. Colvin, William C. Wetsel
      Pages 343-363
  6. Back Matter
    Pages 365-367

About this book


Today, activation endoproteolysis of secretory proteins is recognized as a fundamental biological mechanism of spatial and temporal regulation of protein activity as well as of diversification of protein functions.  In Proprotein Convertases, experts in the field examine detailed methods involving proprotein convertases, the enzymes mediating this endoproteolysis, which reside within or cycle between the various compartments of the secretory pathway.  Providing a timely assessment of impact of activation/inactivation endoproteolysis in the secretory pathway, the volume offers a broader perspective on the biochemistry of the PCSKs (proprotein convertases, subtilisin/kexin-type) by exploring structural and functional analogies with bacterial subtilisin and on the enzymology of endoproteolysis itself by describing the involvement in the process of non-PCSK-type such as cathepsin L.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Meticulous and up-to-date, Proprotein Convertases represents an instructive and useful reference book for all scientists interested in endoproteolytic activation and/or inactivation of secretory proproteins through limited proteolysis, for experts in the field and newcomers to it as well.


Cellular signaling Endoproteinases Endoproteolysis Enzymes Genetically engineered mice PCSKs Phenotyping Secretory proteins

Editors and affiliations

  • Majambu Mbikay
    • 1
  • Nabil G. Seidah
    • 2
  1. 1.Chronic Disease ProgramOttawa Hospital Research InstituteOttawaCanada
  2. 2.(ICRM), Lab. Biochemical NeuroendocrinologyClinical Research Institute of MontrealMontrealCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-203-8
  • Online ISBN 978-1-61779-204-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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