Strain Engineering

Methods and Protocols

  • James A. Williams

Part of the Methods in Molecular Biology book series (MIMB, volume 765)

Table of contents

  1. Front Matter
    Pages i-xi
  2. E. coli

    1. Front Matter
      Pages 1-1
    2. Jindan Zhou, Kenneth E. Rudd
      Pages 3-25
    3. Bong Hyun Sung, Jun Hyoung Lee, Sun Chang Kim
      Pages 43-54
    4. Elie J. Diner, Fernando Garza-Sánchez, Christopher S. Hayes
      Pages 71-82
    5. Chung-Jen Chiang, Po Ting Chen, Shan-Yu Chen, Yun-Peng Chao
      Pages 113-123
    6. Mohan Babu, Alla Gagarinova, Jack Greenblatt, Andrew Emili
      Pages 125-153
  3. Saccharomyces cerevisiae

  4. Strain Engineering Other Industrially Important Microbes

    1. Front Matter
      Pages 295-295
    2. Rahmi Lale, Trygve Brautaset, Svein Valla
      Pages 327-343
    3. Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara
      Pages 345-358
    4. Adam C. Wilson, Hendrik Szurmant
      Pages 359-371
    5. Grace L. Douglas, Yong Jun Goh, Todd R. Klaenhammer
      Pages 373-387
    6. Sarah A. Kuehne, John T. Heap, Clare M. Cooksley, Stephen T. Cartman, Nigel P. Minton
      Pages 389-407
    7. Nobuaki Suzuki, Masayuki Inui, Hideaki Yukawa
      Pages 409-417
    8. Yukari Maezato, Karl Dana, Paul Blum
      Pages 435-445
    9. Jun-ichi Maruyama, Katsuhiko Kitamoto
      Pages 447-456
  5. Back Matter
    Pages 477-480

About this book


Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.


E.coli bacterial genomes chromosomal genes saccharomyces cerevisiae transposon mutagenesis

Editors and affiliations

  • James A. Williams
    • 1
  1. 1.Nature Technology CorporationLincolnUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-196-3
  • Online ISBN 978-1-61779-197-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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