Immune Receptors

Methods and Protocols

  • Jonathan P. Rast
  • James W.D. Booth

Part of the Methods in Molecular Biology book series (MIMB, volume 748)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Elwira Pyż, Gordon D. Brown
    Pages 1-19
  3. Gang Xu, Satoshi Tasumi, Zeev Pancer
    Pages 21-33
  4. Claudine Neyen, Annette Pluddemann, Siamon Gordon
    Pages 35-50
  5. Andrea Stutz, Damien Bertheloot, Eicke Latz
    Pages 69-82
  6. Birte Zurek, Harald Bielig, Thomas A. Kufer
    Pages 107-119
  7. Diane S. Lidke, Shalini T. Low-Nam, Patrick J. Cutler, Keith A. Lidke
    Pages 121-132
  8. Sajedabanu Patel, Ilya Mukovozov, Lisa A. Robinson
    Pages 143-153
  9. Michal Bohdanowicz, Gregory D. Fairn
    Pages 183-193
  10. Alexis J. Torres, David Holowka, Barbara A. Baird
    Pages 195-207
  11. Yunee Kim, Sarah Elschenbroich, Parveen Sharma, Lusia Sepiashvili, Anthony O. Gramolini, Thomas Kislinger
    Pages 227-241
  12. Larry J. Dishaw, M. Gail Mueller, Robert N. Haire, Gary W. Litman
    Pages 243-254
  13. Katherine M. Buckley, Jonathan P. Rast
    Pages 273-298
  14. Back Matter
    Pages 299-301

About this book


Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system.  In Immune Receptors: Methods and Protocols, expert researchers present a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions.  These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to identify novel ligands and methods to analyze downstream receptor signaling, as well as strategies for genomic and proteomic characterization of receptor repertoires. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Immune Receptors: Methods and Protocols offers technical descriptions and protocols that will be useful both to investigators who are interested in carrying out these procedures and to those who seek a deeper understanding of the bench science that lies behind the immunology literature.


Cell biology Downstream signaling Imaging techniques Innate immunity Localization and trafficking Microbial targets Receptor-ligand interactions Recognition proteins

Editors and affiliations

  • Jonathan P. Rast
    • 1
  • James W.D. Booth
    • 2
  1. 1.Sunnybrook Health Sciences CentreUniversity of TorontoTorontoCanada
  2. 2.Sunnybrook Research Institute, Department of ImmunologyUniversity of TorontoTorontoCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-138-3
  • Online ISBN 978-1-61779-139-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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