Receptor Signal Transduction Protocols

Third Edition

  • Gary B. Willars
  • R.A. John Challiss

Part of the Methods in Molecular Biology book series (MIMB, volume 746)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Developing Models for Studying GPCRs

    1. Front Matter
      Pages 1-1
    2. Richard J. Ward, Elisa Alvarez-Curto, Graeme Milligan
      Pages 21-37
    3. Antonio Porcellini, Luisa Iacovelli, Antonio De Blasi
      Pages 39-51
    4. Yan Huang, Gary B. Willars
      Pages 53-84
    5. Alex C. Conner, James Barwell, David R. Poyner, Mark Wheatley
      Pages 85-98
    6. John Davey, Graham Ladds
      Pages 113-131
  3. Examining GPCR Expression and Agonist-Induced Covalent Modifications

    1. Front Matter
      Pages 133-133
    2. David B. Bylund, Myron L. Toews
      Pages 135-164
    3. Trond Brattelid, Finn Olav Levy
      Pages 165-193
    4. Stephen J. Briddon, Barrie Kellam, Stephen J. Hill
      Pages 211-236
    5. Adrian J. Butcher, Andrew B. Tobin, Kok Choi Kong
      Pages 237-249
    6. Adriana Caballero, Adriano Marchese
      Pages 251-259
  4. Examining Early Events in GPCR Signaling

    1. Front Matter
      Pages 261-261
    2. Rajendra Mistry, Mark R. Dowling, R. A. John Challiss
      Pages 263-275
    3. Martin D. Bootman, H. Llewelyn Roderick
      Pages 277-296
    4. Frank Gesellchen, Alessandra Stangherlin, Nicoletta Surdo, Anna Terrin, Anna Zoccarato, Manuela Zaccolo
      Pages 297-316
    5. Michio Nakaya, Mina Ohba, Motohiro Nishida, Hitoshi Kurose
      Pages 317-327
  5. Receptor–Receptor and Receptor–Protein Interactions

    1. Front Matter
      Pages 345-345
    2. Martina Kocan, Kevin D. G. Pfleger
      Pages 357-371
    3. Martin Cottet, Laura Albizu, Laetitia Comps-Agrar, Eric Trinquet, Jean-Philippe Pin, Bernard Mouillac et al.
      Pages 373-387
    4. Avais M. Daulat, Pascal Maurice, Ralf Jockers
      Pages 399-409
    5. Ranju Kumari, Anna Francesconi
      Pages 411-423
    6. James N. Hislop, Mark von Zastrow
      Pages 425-440
  6. Statistical Methods

    1. Front Matter
      Pages 441-441
    2. Domenico Spina
      Pages 443-472
  7. Back Matter
    Pages 473-479

About this book


As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues.  Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and up-to-date, Receptor Signal Transduction Protocols, Third Edition serves the scientific community with a broad collection of the current, practical methodologies in this highly promising field.


Agonist-induced covalent modifications Cell signalling G protein-coupled receptors GPCR expression Receptor-protein interactions Signaling pathways Statistics

Editors and affiliations

  • Gary B. Willars
    • 1
  • R.A. John Challiss
    • 2
  1. 1., Dept. Cell Physiology & PharmacologyUniversity LeicesterLeicesterUnited Kingdom
  2. 2., Dept. Cell Physiology & PharmacologyUniversity LeicesterLeicesterUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-125-3
  • Online ISBN 978-1-61779-126-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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