RNAi and Plant Gene Function Analysis

Methods and Protocols

  • Hiroaki Kodama
  • Atsushi Komamine

Part of the Methods in Molecular Biology book series (MIMB, volume 744)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yuichiro Watanabe
    Pages 1-11
  3. Muthappa Senthil-Kumar, Kirankumar S. Mysore
    Pages 13-25
  4. Frédéric Masclaux, Jean-Philippe Galaud
    Pages 37-55
  5. Basel Khraiwesh, Isam Fattash, M. Asif Arif, Wolfgang Frank
    Pages 57-79
  6. Cai-Zhong Jiang, Jen-Chih Chen, Michael Reid
    Pages 81-96
  7. Jutta Maria Helm, Elena Dadami, Kriton Kalantidis
    Pages 97-108
  8. Ha-il Jung, Zhiyang Zhai, Olena K. Vatamaniuk
    Pages 109-127
  9. Toshiyuki Fukuhara, Syunichi Urayama, Ryo Okada, Eri Kiyota, Hiromitsu Moriyama
    Pages 129-144
  10. Erika Varkonyi-Gasic, Roger P. Hellens
    Pages 145-157
  11. William P. Donovan, Yuanji Zhang, Miya D. Howell
    Pages 159-173
  12. Ying-Hsuan Sun, Shanfa Lu, Rui Shi, Vincent L. Chiang
    Pages 175-186
  13. Nazmul Haque, Masamichi Nishiguchi
    Pages 187-197
  14. Sang Yeol Lee, Kyun Oh Lee
    Pages 211-224
  15. Tomoya Asano, Takumi Nishiuchi
    Pages 225-233
  16. Back Matter
    Pages 235-244

About this book


The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.


Functional Genomics Studies Gene Silencing Plant Biology Plant Engineering RNA Sequencing RNA Transfer RNAi Technology

Editors and affiliations

  • Hiroaki Kodama
    • 1
  • Atsushi Komamine
    • 2
  1. 1.Graduate School of HorticultureChiba UniversityChibaJapan
  2. 2.Evolutionary BiologyResearch Institute ofTokyo, SetagayaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-122-2
  • Online ISBN 978-1-61779-123-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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