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RNA Interference Techniques

  • Scott Q. Harper

Part of the Neuromethods book series (NM, volume 58)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Ryan L. Boudreau, Sara E. Garwick-Coppens, Jian Liu, Lindsay M. Wallace, Scott Q. Harper
    Pages 19-37
  3. Kevin D. Foust, Brian K. Kaspar
    Pages 59-76
  4. Eric J. Devor, Lingyan Huang
    Pages 77-90
  5. Jinmai Jiang, Eun Joo Lee, Melissa G. Piper, Clay B. Marsh, Thomas D. Schmittgen
    Pages 113-121
  6. Markus Hirsch, Il-Han Kim, Anne Järve, Roger Fischer, Michael F. Trendelenburg, Ulrich Massing et al.
    Pages 141-157
  7. Lu Ping Tan, Anke van den Berg, Joost L. Kluiver
    Pages 159-169
  8. Back Matter
    Pages 171-173

About this book

Introduction

Non-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi).  To date, hundreds of conserved and species-specific microRNAs have been identified in organisms ranging from single-celled algae to humans.  Many of these tiny RNAs are now known to play fundamental roles in developmental biology and disease pathogenesis.  In addition, RNAi has emerged as a technology useful for manipulating gene expression.  In RNA Interference Techniques, expert researchers present detailed methods for designing and delivering artificial inhibitory RNAs to neural tissue and for detecting or cloning endogenous microRNAs, all in order to aid investigators’ attempts to ask basic biological questions or develop therapeutics for dominant neurogenetic disorders, cancer, or viral infection. As a volume in the successful Neuromethods series, the chapters provide authoritative accounts of the most commonly used approaches in the field today.

 

Cutting-edge and concise, RNA Interference Techniques promises to support the vital research in the field of RNAi and miRNAs, ever-continuing to grow rapidly and gain increasing importance in basic and translational biology.

Keywords

Artificial inhibitory RNAs Designer inhibitory RNAs Detecting or cloning Disease pathogenesis Gene expression MicroRNAs RNAi Therapeutics

Editors and affiliations

  • Scott Q. Harper
    • 1
  1. 1.Research Institute, Center for Gene TherapyNationwide Children’s HospitalColumbusUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-114-7
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-113-0
  • Online ISBN 978-1-61779-114-7
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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