Atomic Force Microscopy in Biomedical Research

Methods and Protocols

  • Pier Carlo Braga
  • Davide Ricci

Part of the Methods in Molecular Biology book series (MIMB, volume 736)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. The Basics of Atomic Force Microscopy

    1. Front Matter
      Pages 1-1
    2. Bruno Torre, Davide Ricci, Pier Carlo Braga
      Pages 3-18
    3. Bruno Torre, Claudio Canale, Davide Ricci, Pier Carlo Braga
      Pages 19-29
    4. Claudio Canale, Bruno Torre, Davide Ricci, Pier Carlo Braga
      Pages 31-43
  3. Molecule Imaging

    1. Front Matter
      Pages 45-45
    2. Stewart M. Carnally, J. Michael Edwardson, Nelson P. Barrera
      Pages 47-60
    3. Claudio Canale, Annalisa Relini, Alessandra Gliozzi
      Pages 81-95
    4. Maria Gaczynska, Pawel A. Osmulski
      Pages 117-132
    5. Bradley E. Layton, M. Brent Boyd
      Pages 133-151
  4. Nanoscale Surface Analysis and Cell Imaging

    1. Front Matter
      Pages 169-169
    2. Alexander McPherson, Yurii G. Kuznetsov
      Pages 171-195
    3. Christian Rankl, Linda Wildling, Isabel Neundlinger, Ferry Kienberger, Hermann Gruber, Dieter Blaas et al.
      Pages 197-210
    4. Wanderley de Souza, Gustavo M. Rocha, Kildare Miranda, Paulo M. Bisch, Gilberto Weissmuller
      Pages 211-221
    5. Andreas Ebner, Hermann Schillers, Peter Hinterdorfer
      Pages 223-241
    6. Davide Ricci, Massimo Grattarola, Mariateresa Tedesco
      Pages 243-257
    7. Narahari V. Joshi, Ibis Cruz, Jesus A. Osuna
      Pages 259-284
    8. Takayuki Uchihashi, Toshio Ando
      Pages 285-300
  5. Non-topographical Applications (Force-Spectroscopy)

    1. Front Matter
      Pages 301-301
    2. Félix Rico, Calvin Chu, Vincent T. Moy
      Pages 331-353
    3. Martin Benoit, Christine Selhuber-Unkel
      Pages 355-377
    4. Ting Cao, Haiying Tang, Xuemei Liang, Anfeng Wang, Gregory W. Auner, Steven O. Salley et al.
      Pages 379-388
  6. Investigating Drug Action

  7. Atomic Force Microscopy as a Nanotool

    1. Front Matter
      Pages 437-437
    2. Miklós S. Z. Kellermayer
      Pages 439-456
    3. Régis Barattin, Normand Voyer
      Pages 457-483
    4. Ning Xi, Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Donna H. Wang, Kristina Seiffert-Sinha et al.
      Pages 485-503
  8. Back Matter
    Pages 505-508

About this book


With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life.  Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use.  Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies.


AFM Chemical structural information Drug action Molecular imaging Nanobiology Nanoscale surface analysis Nanotools Spectroscopy

Editors and affiliations

  • Pier Carlo Braga
    • 1
  • Davide Ricci
    • 2
  1. 1.School of Medicine, Department of PharmacologyUniversity of MilanMilanoItaly
  2. 2., Robotics, Brain & Cognitive SciencesItalian Institute of TechnologyGenovaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-104-8
  • Online ISBN 978-1-61779-105-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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