Mammalian Chromosome Engineering

Methods and Protocols

  • Gyula Hadlaczky

Part of the Methods in Molecular Biology book series (MIMB, volume 738)

Table of contents

  1. Front Matter
    Pages i-ix
  2. Olivia Hibbitt, Richard Wade-Martins
    Pages 19-40
  3. Marianna Paulis
    Pages 57-67
  4. Ivana Grabundzija, Zsuzsanna Izsvák, Zoltán Ivics
    Pages 69-85
  5. Peter Blazso, Ildiko Sinko, Robert L. Katona
    Pages 161-181
  6. Back Matter
    Pages 239-257

About this book


The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry.  Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system.  Written in the highly successful Methods in Molecular Biology™ series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.


De novo platforms Engineered chromosomes Extrachromosomal vectors Gene technology Minichromosome manipulation Pharmaceutical protein production Synthetic artificial chromosomes Transgenic animals Transposon systems

Editors and affiliations

  • Gyula Hadlaczky
    • 1
  1. 1.Biological Research Center, Institute of GeneticsHungarian Academy of SciencesSzegedHungary

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-098-0
  • Online ISBN 978-1-61779-099-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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