High-Throughput Next Generation Sequencing

Methods and Applications

  • Young Min Kwon
  • Steven C. Ricke

Part of the Methods in Molecular Biology book series (MIMB, volume 733)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Genome Sequencing

    1. Front Matter
      Pages 1-1
    2. Kathleen E. Steinmann, Christopher E. Hart, John F. Thompson, Patrice M. Milos
      Pages 3-24
  3. Gene Expression Analysis

    1. Front Matter
      Pages 35-35
    2. Tal Raz, Marie Causey, Daniel R. Jones, Alix Kieu, Stan Letovsky, Doron Lipson et al.
      Pages 37-49
    3. Fatih Ozsolak, Patrice M. Milos
      Pages 51-61
    4. Jane M. Liu, Andrew Camilli
      Pages 63-79
  4. Microbial Diversity

    1. Front Matter
      Pages 105-105
    2. Husen Zhang, Prathap Parameswaran, Jonathan Badalamenti, Bruce E. Rittmann, Rosa Krajmalnik-Brown
      Pages 107-128
    3. John Schellenberg, Matthew G. Links, Janet E. Hill, Sean M. Hemmingsen, Geoffrey A. Peters, Tim J. Dumonceaux
      Pages 143-158
  5. Metagenomics

    1. Front Matter
      Pages 171-171
    2. Jack A. Gilbert, Bonnie Laverock, Ben Temperton, Simon Thomas, Martin Muhling, Margaret Hughes
      Pages 173-183
    3. Taejoong Kim, Egbert Mundt
      Pages 185-194
    4. Jack A. Gilbert, Margaret Hughes
      Pages 195-205
  6. Sequence Profiling for Functional Analysis

    1. Front Matter
      Pages 207-207
    2. Sandy M. S. Wong, Jeffrey D. Gawronski, David Lapointe, Brian J. Akerley
      Pages 209-222
    3. Suhua Feng, Liudmilla Rubbi, Steven E. Jacobsen, Matteo Pellegrini
      Pages 223-238
  7. Sequencing Library Preparation

  8. Back Matter
    Pages 299-308

About this book


Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes.  In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.


Biological systems Complex molecular events DNA sequences Functional analysis Gene expression analysis Library preparation Metagenomics Microbial communities Microbial diversity Microorganisms

Editors and affiliations

  • Young Min Kwon
    • 1
  • Steven C. Ricke
    • 2
  1. 1., Department of Poultry ScienceUniversity of ArkansasFayettevilleUSA
  2. 2.Department of Food ScienceUniversity of ArkansasFayettevilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-088-1
  • Online ISBN 978-1-61779-089-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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