Argonaute Proteins

Methods and Protocols

  • Tom C. Hobman
  • Thomas F. Duchaine

Part of the Methods in Molecular Biology book series (MIMB, volume 725)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Thomas A. Volpe, Jessica DeMaio
    Pages 15-28
  3. Keita Miyoshi, Tomoko N. Okada, Haruhiko Siomi, Mikiko C. Siomi
    Pages 29-43
  4. Bing Yao, Songqing Li, Shang Li Lian, Marvin J. Fritzler, Edward K. L. Chan
    Pages 45-62
  5. Julia Stoehr, Gunter Meister
    Pages 77-90
  6. Tomoko Kawamata, Yukihide Tomari
    Pages 91-105
  7. Marjorie P. Perron, Patricia Landry, Isabelle Plante, Patrick Provost
    Pages 121-141
  8. Jing Li, Nima Najand, Wendy Long, Andrew Simmonds
    Pages 143-159
  9. Justin M. Pare, Joaquin Lopez-Orozco, Tom C. Hobman
    Pages 161-172
  10. Lydia V. McClure, Gil Ju Seo, Christopher S. Sullivan
    Pages 173-189
  11. Stephanie Eckhardt, Emilia Szostak, Zhaolin Yang, Ramesh Pillai
    Pages 191-206
  12. Marc R. Fabian, Yuri V. Svitkin, Nahum Sonenberg
    Pages 207-217
  13. Guillaume Jannot, Alejandro Vasquez-Rifo, Martin J. Simard
    Pages 233-249
  14. Weifeng Gu, Julie M. Claycomb, Pedro J. Batista, Craig C. Mello, Darryl Conte
    Pages 251-280
  15. Yohei Kirino, Anastassios Vourekas, Eugene Khandros, Zissimos Mourelatos
    Pages 281-293

About this book

Introduction

With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner.  Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels.  In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.

Keywords

Gene expression Gene silencing Molecular therapeutics RNA interference RNA-binding proteins RNAi Yeast and mammalian systems

Editors and affiliations

  • Tom C. Hobman
    • 1
  • Thomas F. Duchaine
    • 2
  1. 1., Department of Cell BiologyUniversity of AlbertaEdmontonCanada
  2. 2.Goodman Cancer Research Centre, Department of BiochemistryMcGill UniversityMontrealCanada

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-046-1
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-045-4
  • Online ISBN 978-1-61779-046-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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