Argonaute Proteins

Methods and Protocols

  • Tom C. Hobman
  • Thomas F. Duchaine

Part of the Methods in Molecular Biology book series (MIMB, volume 725)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Thomas A. Volpe, Jessica DeMaio
    Pages 15-28
  3. Keita Miyoshi, Tomoko N. Okada, Haruhiko Siomi, Mikiko C. Siomi
    Pages 29-43
  4. Bing Yao, Songqing Li, Shang Li Lian, Marvin J. Fritzler, Edward K. L. Chan
    Pages 45-62
  5. Julia Stoehr, Gunter Meister
    Pages 77-90
  6. Tomoko Kawamata, Yukihide Tomari
    Pages 91-105
  7. Marjorie P. Perron, Patricia Landry, Isabelle Plante, Patrick Provost
    Pages 121-141
  8. Jing Li, Nima Najand, Wendy Long, Andrew Simmonds
    Pages 143-159
  9. Justin M. Pare, Joaquin Lopez-Orozco, Tom C. Hobman
    Pages 161-172
  10. Lydia V. McClure, Gil Ju Seo, Christopher S. Sullivan
    Pages 173-189
  11. Stephanie Eckhardt, Emilia Szostak, Zhaolin Yang, Ramesh Pillai
    Pages 191-206
  12. Marc R. Fabian, Yuri V. Svitkin, Nahum Sonenberg
    Pages 207-217
  13. Guillaume Jannot, Alejandro Vasquez-Rifo, Martin J. Simard
    Pages 233-249
  14. Weifeng Gu, Julie M. Claycomb, Pedro J. Batista, Craig C. Mello, Darryl Conte
    Pages 251-280
  15. Yohei Kirino, Anastassios Vourekas, Eugene Khandros, Zissimos Mourelatos
    Pages 281-293
  16. Back Matter
    Pages 333-344

About this book


With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner.  Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels.  In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.


Gene expression Gene silencing Molecular therapeutics RNA interference RNA-binding proteins RNAi Yeast and mammalian systems

Editors and affiliations

  • Tom C. Hobman
    • 1
  • Thomas F. Duchaine
    • 2
  1. 1., Department of Cell BiologyUniversity of AlbertaEdmontonCanada
  2. 2.Goodman Cancer Research Centre, Department of BiochemistryMcGill UniversityMontrealCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-045-4
  • Online ISBN 978-1-61779-046-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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