Protein Microarray for Disease Analysis

Methods and Protocols

  • Catherine J. Wu

Part of the Methods in Molecular Biology book series (MIMB, volume 723)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Protein-Detecting Analytical Microarrays

    1. Front Matter
      Pages 1-1
    2. Jochen M. Schwenk, Peter Nilsson
      Pages 29-36
    3. Víctor J. Cid, Ekkehard Kauffmann, María Molina
      Pages 37-55
    4. Daniel C. Propheter, Ku-Lung Hsu, Lara K. Mahal
      Pages 67-77
  3. Antigen Microarrays for Immunoprofiling

    1. Front Matter
      Pages 79-79
    2. Persis P. Wadia, Bita Sahaf, David B. Miklos
      Pages 81-104
    3. Dirk F. H. Winkler, Heiko Andresen, Kai Hilpert
      Pages 105-127
    4. Robert J. Caiazzo Jr., Dennis J. O’Rourke, Timothy J. Barder, Bryce P. Nelson, Brian C.-S. Liu
      Pages 129-148
    5. Sahar Sibani, Joshua LaBaer
      Pages 149-161
  4. Protein Function Microarrays

    1. Front Matter
      Pages 163-163
    2. Michael G. Smith, Jason Ptacek, Michael Snyder
      Pages 201-212
  5. Strategies for Validation of Candidate Targets

    1. Front Matter
      Pages 225-225
    2. Ovidiu Marina, Jonathan S. Duke-Cohan, Catherine J. Wu
      Pages 239-254
  6. Generation of Proteomic Libraries

    1. Front Matter
      Pages 255-255
    2. Charles Yu, Kenneth H. Wan, Ann S. Hammonds, Mark Stapleton, Joseph W. Carlson, Susan E. Celniker
      Pages 257-272
  7. Detection Methods

    1. Front Matter
      Pages 273-273
    2. Rosa I. Gallagher, Alessandra Silvestri, Emanuel F. Petricoin III, Lance A. Liotta, Virginia Espina
      Pages 275-301
    3. Christopher Lausted, Zhiyuan Hu, Leroy Hood
      Pages 321-333
  8. Data Analysis Techniques for Protein Function Microarrays

    1. Front Matter
      Pages 335-335
    2. David S. DeLuca, Ovidiu Marina, Surajit Ray, Guang Lan Zhang, Catherine J. Wu, Vladimir Brusic
      Pages 337-347
    3. Guang Lan Zhang, David S. DeLuca, Vladimir Brusic
      Pages 349-364
  9. Back Matter
    Pages 365-373

About this book


Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response.  In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease.  These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes.  Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Practical and cutting-edge,  Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.


Antigen microarrays Biochemistry Candidate target validation Data analysis Disease biomarkers Drug development Humoral immune response Immunoprofiling Protein-protein interactions Proteomics

Editors and affiliations

  • Catherine J. Wu
    • 1
  1. 1.Cancer Vaccine Center, Department of Medical OncologyDana-Farber Cancer InstituteBostonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2011
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-042-3
  • Online ISBN 978-1-61779-043-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Consumer Packaged Goods